Miki Kota, Fujieda Hiroaki, Ueno Yoshiyuki, Arakane Toru, Fujii Yutaka
Medical Engineering Center, Shimane University Hospital, Enya-cho 89-1, Izumo-shi 693-8501, Shimane, Japan.
Graduate School, Niigata University of Health and Welfare, Shimamicho1398, Kitaku 950-3198, Niigata, Japan.
J Clin Med. 2025 Mar 2;14(5):1686. doi: 10.3390/jcm14051686.
Cardiopulmonary bypass (CPB) and extracorporeal membrane oxygenation (ECMO) are widely used. Previous methods to reduce inflammation have shown inconsistent results. We developed a cytokine adsorption column using polymethyl methacrylate (PMMA) and investigated its anti-inflammatory effects during ECMO. Male Sprague-Dawley rats were divided into three groups (seven rats in each group): SHAM, ECMO, and ECMO with PMMA (PMMA group). Experiments comprised 180 min of cannulation only in the SHAM group and 60 min of ECMO followed by 120 min of observation in the ECMO and PMMA groups. PMMA adsorption was conducted from 30 min after ECMO initiation to completion in the PMMA group. Blood parameters and cytokines were measured during experiments. Lung tissues were collected after the experiment for evaluation of tissue edema. The PMMA group showed significantly lower levels of tumor necrosis factor alpha (TNF-α) and interleukin(IL)-6 compared to the ECMO group at 120 min after completing ECMO. However, there were no significant differences in IL-10 levels between the ECMO group and the PMMA group at the same time points. Lung edema incidence was significantly lower in the PMMA group. The PMMA column effectively suppressed systemic inflammatory reactions during ECMO.
体外循环（CPB）和体外膜肺氧合（ECMO）被广泛应用。以往减轻炎症的方法效果并不一致。我们研发了一种使用聚甲基丙烯酸甲酯（PMMA）的细胞因子吸附柱，并研究了其在ECMO期间的抗炎作用。将雄性Sprague-Dawley大鼠分为三组（每组七只）：假手术组（SHAM）、ECMO组和使用PMMA的ECMO组（PMMA组）。实验中，SHAM组仅插管180分钟，ECMO组和PMMA组进行60分钟的ECMO，随后观察120分钟。在PMMA组，从ECMO开始后30分钟至结束进行PMMA吸附。实验期间测量血液参数和细胞因子。实验结束后收集肺组织评估组织水肿情况。与ECMO组相比，在完成ECMO后120分钟时，PMMA组的肿瘤坏死因子α（TNF-α）和白细胞介素（IL）-6水平显著降低。然而，在相同时间点，ECMO组和PMMA组的IL-10水平无显著差异。PMMA组的肺水肿发生率显著更低。PMMA柱在ECMO期间有效抑制了全身炎症反应。
