El-Kaliuoby Mai I, Morsy Ashraf, Abdel-Salam Ahmed H, Morsy Ahmed, El-Khatib Ahmed M, Khalil Alaa M
Physics and Chemistry Department, Faculty of Education, Alexandria University, Alexandria, 21544, Egypt.
Faculty of Engineering, Petrochemical Department, Pharos University in Alexandria, Canal El Mahmoudia Street, Beside Green Plaza Complex 21648, Alexandria, Egypt.
Sci Rep. 2025 Mar 19;15(1):9524. doi: 10.1038/s41598-025-91408-y.
The urge need for innovative integration between Electromagnetic Waves (EMWs) and nanotechnology offers exciting possibilities for improving antimicrobial treatments to combat antibacterial resistant bacterial infections. This study explores how EMWs at range below 300 Hz can enhance the antibacterial efficacy of Graphene Oxide Nanoparticles (GONPs) against Pseudomonas aeruginosa, a significant pathogen in antibiotic resistance. EMWs at range below 300 Hz, interact with bacterial cell membranes to affect ion channels, permeability, and cellular signaling, offering a non-invasive method to amplify antimicrobial effects. GONPs synthesized through glucose pyrolysis and characterized by X-ray diffraction, UV-visible spectroscopy, high-resolution transmission electron microscopy, and Fourier-transform infrared spectroscopy, exhibit potent antibacterial properties due to their sharp edges, large surface area, and ability to generate Reactive Oxygen Species (ROS). These nanoparticles disrupt bacterial membranes, form biofilms, and damage cellular components through oxidative stress. The study examines how those EMWs can enhance GONP penetration into bacterial cells, increase ROS production, and disrupt biofilms. By optimizing EMWs parameters such as frequency, intensity, and duration this research aims to develop new, non-invasive antibacterial therapies. The results could lead to advanced antimicrobial strategies, integrating nanotechnology with electromagnetic field exposure, offering innovative solutions to address antibiotic-resistant infections and improve treatment efficacy. This approach represents a significant step toward more effective, targeted antibacterial therapies.
迫切需要将电磁波（EMW）与纳米技术进行创新性整合，这为改进抗菌治疗以对抗耐抗菌细菌感染提供了令人兴奋的可能性。本研究探讨了频率低于300Hz的电磁波如何增强氧化石墨烯纳米颗粒（GONP）对铜绿假单胞菌的抗菌效果，铜绿假单胞菌是抗生素耐药性方面的一种重要病原体。频率低于300Hz的电磁波与细菌细胞膜相互作用，影响离子通道、通透性和细胞信号传导，提供了一种非侵入性方法来增强抗菌效果。通过葡萄糖热解合成并通过X射线衍射、紫外可见光谱、高分辨率透射电子显微镜和傅里叶变换红外光谱表征的GONP，由于其尖锐边缘、大表面积和产生活性氧（ROS）的能力而具有强大的抗菌性能。这些纳米颗粒破坏细菌膜、形成生物膜并通过氧化应激损害细胞成分。该研究考察了这些电磁波如何增强GONP对细菌细胞的渗透、增加ROS产生并破坏生物膜。通过优化诸如频率、强度和持续时间等电磁波参数，本研究旨在开发新的非侵入性抗菌疗法。研究结果可能会带来先进的抗菌策略，将纳米技术与电磁场暴露相结合，为解决抗生素耐药性感染和提高治疗效果提供创新解决方案。这种方法代表了朝着更有效、有针对性的抗菌疗法迈出的重要一步。
