Braitenbach Beatriz Paim de Figueiredo, Cerqueira Carla Barreto Silva de, Lima Sarah Souza, Hegouet Isadora de Carvalho, Moreno Bruna Carvalho Lopez, Medrado Alena Ribeiro Alves Peixoto
Federal University of Bahia, Salvador, Brazil.
Bahiana School of Medicine and Public Health, Salvador, Brazil.
Lasers Med Sci. 2025 Mar 20;40(1):153. doi: 10.1007/s10103-025-04410-1.
Adipocyte infiltration consists of a temporary increase in the number of adipocytes in the microenvironment of tissue injury. There is already evidence in the literature of adipocytes' participation in promoting the inflammatory response, and parallelly laser photobiomodulation can benefit the tissue repair process. This study aimed to chronologically analyze adipocytic infiltration in the repair of photobiomodulated skin wounds experimentally induced in rats through histomorphometric analysis. The sample consisted of 20 rats divided into 2 groups: control group and group subjected to laser photobiomodulation. The skin portions of the back of rats were processed and stained with Hematoxylin-Eosin in 4 μm thick sections including the surgical wound 5 and 10 days after the proposed treatments. Qualitative and quantitative analyses were performed by capturing images of tissue sections, describing the organizational pattern of adipocytes around the surgical wound and counting individual adipocytes in the connective tissue in formation. Adipocytic infiltration was observed in both experimental groups on the 5th day, with a decrease on the 10th day. The group treated with photobiomodulation presented a greater number of adipocytes compared to the control group, in both periods analyzed. The findings of the present study seem to corroborate the literature, which indicates that adipose cells might stimulate inflammation and repair, and photobiomodulation can enhance these effects, since it aids the process of adipocytic infiltration in the injured area. Clinical trial number: Not applicable.
脂肪细胞浸润是指组织损伤微环境中脂肪细胞数量的暂时增加。文献中已有证据表明脂肪细胞参与促进炎症反应，同时激光光生物调节可有利于组织修复过程。本研究旨在通过组织形态计量学分析，按时间顺序分析大鼠实验性诱导的光生物调节皮肤伤口修复过程中的脂肪细胞浸润情况。样本包括20只大鼠，分为2组：对照组和接受激光光生物调节的组。在拟定治疗后5天和10天，对大鼠背部皮肤部分进行处理，制成4μm厚的切片并用苏木精-伊红染色，包括手术伤口。通过拍摄组织切片图像、描述手术伤口周围脂肪细胞的组织模式以及计数正在形成的结缔组织中的单个脂肪细胞进行定性和定量分析。在第5天，两个实验组均观察到脂肪细胞浸润，在第10天有所减少。在分析的两个时期内，与对照组相比，接受光生物调节治疗的组脂肪细胞数量更多。本研究结果似乎证实了文献报道，即脂肪细胞可能刺激炎症和修复，而光生物调节可增强这些作用，因为它有助于受伤区域的脂肪细胞浸润过程。临床试验编号：不适用。
