Huang Wei, Zheng Hongying, Wu Jiaming, Wang Lingfei, Li Huaqiong, Wen Feng, Chen Congde
Department of Pediatric Surgery, The 2nd Affiliated Hospital and Yuying Children's Hospital of WMU, Wenzhou, China.
Key Laboratory of Structural Malformations in Children of Zhejiang Province, Wenzhou, China.
Transl Androl Urol. 2025 Feb 28;14(2):266-279. doi: 10.21037/tau-24-598. Epub 2025 Feb 25.
In urethral reconstruction, autologous tissue grafts are subject to various limitations. Decellularized tissue has attracted significant interest in tissue engineering (TE) and regenerative medicine due to great biocompatibility and bioactivity. We investigated the effect of decellularized tilapia skin (DTS) in a rabbit urethral defect model to explore its feasibility and effectiveness as a TE construction for urethral reconstruction.
Fresh tilapia skins were decellularized and verified through its residual deoxyribonucleic acid (DNA) content measurement. The physical properties and degradation profile of DTS were characterized as well. Finally, we established a rabbit urethral defect model and evaluated the effect of DTS on urethral defect healing through pathological examination and immunohistochemistry (IHC) staining.
The residual DNA content in the DTS was 32.94 ng/mg. Electron microscopy showed that the DTS retained its three-dimensional structure. , degradation experiments showed that DTS degraded at a faster rate than that of small intestinal submucosa (SIS). After implantation of the DTS, the penile tissue was well repaired. In the early stage of tissue repair, the tissue was gradually replaced by new collagen. In addition, smooth muscle content was significantly increased. No significant immune rejection was observed in the tissues during the repair process and the inflammatory response was significantly milder than other group. Nevertheless, angiogenesis markers, the numbers of blood vessels and blood vessel area in DTS intervention groups were the highest at 4 weeks post-implantation.
DTS could degrade gradually during urethral reconstruction and demonstrated its better biocompatibility in terms of tissue morphology, microanatomy of tissues, severity of inflammation, collagen deposition and angiogenesis in defect region its match control groups. As an excellent TE material, it is expected to be used in clinical urethral reconstruction in future.
在尿道重建中，自体组织移植物存在各种局限性。去细胞组织因其良好的生物相容性和生物活性，在组织工程（TE）和再生医学中引起了广泛关注。我们在兔尿道缺损模型中研究了去细胞罗非鱼皮（DTS）的作用，以探讨其作为尿道重建组织工程构建物的可行性和有效性。
将新鲜罗非鱼皮进行去细胞处理，并通过测量其残余脱氧核糖核酸（DNA）含量进行验证。还对DTS的物理性质和降解情况进行了表征。最后，我们建立了兔尿道缺损模型，并通过病理检查和免疫组织化学（IHC）染色评估DTS对尿道缺损愈合的影响。
DTS中的残余DNA含量为32.94 ng/mg。电子显微镜显示DTS保留了其三维结构。降解实验表明，DTS的降解速度比小肠黏膜下层（SIS）快。植入DTS后，阴茎组织得到良好修复。在组织修复早期，组织逐渐被新的胶原蛋白替代。此外，平滑肌含量显著增加。在修复过程中，组织未观察到明显的免疫排斥反应，炎症反应明显轻于其他组。然而，在植入后4周时，DTS干预组的血管生成标志物、血管数量和血管面积最高。
在尿道重建过程中，DTS可逐渐降解，并且在组织形态、组织微观解剖、炎症严重程度、缺损区域的胶原沉积和血管生成方面，与对照组织相比，显示出更好的生物相容性。作为一种优良的组织工程材料，有望在未来用于临床尿道重建。
