Lin Yingying, Hou Jiaxin, Li Bin, Shu Weikang, Wan Jingjing
School of Chemistry and Molecular Engineering, East China Normal University, Shanghai, 200241, P. R. China.
ACS Nano. 2025 Apr 1;19(12):11604-11624. doi: 10.1021/acsnano.4c18470. Epub 2025 Mar 24.
Spatial omics is emerging as a focus of life sciences because of its applications in investigating the molecular mechanisms of cancer, mapping cellular distributions, and revealing specific cellular ecological niches. Notably, the in-depth acquisition of spatial omics information relies on highly sensitive, high-resolution, and high-throughput biological analysis tools and techniques. However, conventional methods of omics data acquisition still suffer from some drawbacks such as limited-resolution and low-throughput and are difficult to adapt directly to the collection of high-quality spatial omics data. Recently, an increasing number of advanced nanomaterials and molecular probes are employed in spatial omics due to their excellent optoelectronic properties, biocompatibility, and multifunction. These well-designed innovative nanoscaffolds successfully enhance the key parameters of spatial omics and, thus, increase the spatial resolution, detection sensitivity, and detection throughput. This review summarizes the design and application of functional nanoscaffolds for spatial omics in recent years, with a particular emphasis on nanomaterials and molecular probes. We believe that the present review can inspire and motivate researchers in designing and selecting appropriate materials and probes for high-quality spatial omics, thus promoting the development of spatial omics and life sciences.
空间组学因其在研究癌症分子机制、绘制细胞分布图以及揭示特定细胞生态位方面的应用，正逐渐成为生命科学的一个焦点。值得注意的是，深入获取空间组学信息依赖于高灵敏度、高分辨率和高通量的生物分析工具及技术。然而，传统的组学数据获取方法仍存在一些缺点，如分辨率有限、通量低，难以直接适用于高质量空间组学数据的采集。近年来，由于其优异的光电性能、生物相容性和多功能性，越来越多的先进纳米材料和分子探针被应用于空间组学。这些精心设计的创新纳米支架成功地提升了空间组学的关键参数，从而提高了空间分辨率、检测灵敏度和检测通量。本综述总结了近年来用于空间组学的功能性纳米支架的设计与应用，特别强调了纳米材料和分子探针。我们相信，本综述能够启发和激励研究人员设计和选择合适的材料及探针用于高质量的空间组学研究，从而推动空间组学和生命科学的发展。
