Liu Yu, Wei Zeyu, Duan Rui, Wang Ke, Xu Tianyue, Mao Binxian, Zhang Junjie
Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, 59 Cangwu Road, Lianyungang 222005, China.
College of Marine Food and Bioengineering, Jiangsu Ocean University, 59 Cangwu Road, Lianyungang 222005, China.
J Funct Biomater. 2025 Feb 25;16(3):81. doi: 10.3390/jfb16030081.
Acellular tissue matrices of fish skin origin are highly promising materials for tissue engineering due to their low biological risks and few religious restrictions. The main component of acellular fish skin matrices (AFSMs) is collagen, but collagen properties significantly differ between marine and freshwater fish. Although the characteristics of acellular matrices may vary, relevant reports about them are few. In this study, we used cod and tilapia fish skin as raw materials to prepare acellular matrices with low DNA content (≤50 ng/mg) and low endotoxin. They were denoted as C-AFSM (cod) and T-AFSM (tilapia) and had endotoxin removal rates of 92.47% and 96.73%, respectively. Their physicochemical properties, cytotoxicity, and wound healing effects were evaluated and compared. Scanning electron microscopy images showed that C-AFSM and T-AFSM had collagenous meshwork and high porosity. They also did not induce skin irritations. Their proliferation rates on mouse fibroblasts at 36 h were 192.21% ± 33.25% and 162.89% ± 36.47%, respectively. The wound healing effect of C-AFSM was faster than that of T-AFSM group (7 and 14 days: 45.3% ± 5.99% and 93.77% ± 1.58% for C-AFSM and 39.7% ± 2.84% and 93.35% ± 1.1% for T-AFSM, respectively). Therefore, the two acellular fish skin matrices can be used as tissue-engineering materials for wound repair, with C-AFSM being more effective than T-AFSM.
鱼皮来源的脱细胞组织基质因其低生物风险和较少的宗教限制，是组织工程中极具前景的材料。脱细胞鱼皮基质（AFSMs）的主要成分是胶原蛋白，但海洋鱼类和淡水鱼类的胶原蛋白特性存在显著差异。尽管脱细胞基质的特性可能有所不同，但相关报道较少。在本研究中，我们以鳕鱼和罗非鱼鱼皮为原料，制备了低DNA含量（≤50 ng/mg）和低内毒素的脱细胞基质。它们分别被标记为C-AFSM（鳕鱼）和T-AFSM（罗非鱼），内毒素去除率分别为92.47%和96.73%。对它们的理化性质、细胞毒性和伤口愈合效果进行了评估和比较。扫描电子显微镜图像显示，C-AFSM和T-AFSM具有胶原网络结构和高孔隙率。它们也不会引起皮肤刺激。它们在36小时时对小鼠成纤维细胞的增殖率分别为192.21%±33.25%和162.89%±36.47%。C-AFSM的伤口愈合效果比T-AFSM组更快（7天和14天时：C-AFSM分别为45.3%±5.99%和93.77%±1.58%，T-AFSM分别为39.7%±2.84%和93.35%±1.1%）。因此，这两种脱细胞鱼皮基质可用作伤口修复的组织工程材料，其中C-AFSM比T-AFSM更有效。
