Guerra-López José R, Bianchi Ana E, Ramos Marcelo A, Ubertino Mauro, Ferraresi-Curotto Verónica, Güida Jorge A, Barbaro Katia, Zhukova Anna A, Grigorieva Victoria Yu, Rau Julietta V, Echeverría Gustavo A
Departamento de Ciencias Básicas, Universidad Nacional de Luján, Ruta 5 y 7, Luján 6700, Argentina.
Instituto de Ecología y Desarrollo Sustentable (INEDES, CONICET-UNLu), Luján 6700, Argentina.
J Funct Biomater. 2025 Mar 4;16(3):88. doi: 10.3390/jfb16030088.
Bacterial resistance and the demand for novel antibacterial strategies represent major challenges in contemporary medicine. In this study, zinc-doped hydroxyapatite (Zn-HA) samples with 3, 5, and 10 wt% Zn(II) were synthesized using wet precipitation synthesis and sintered at 700 and 800 °C. The samples were characterized by X-ray Diffraction, Fourier Transform Infrared Spectroscopy, Raman Spectroscopy, and Scanning Electron Microscopy. The antimicrobial properties of the Zn-HA were tested against four bacterial strains-, , , -and the fungus Candida albicans. Both 5 wt% and 10 wt% Zn-HA effectively inhibited the growth of all microorganisms. Notably, 10wt% Zn-HA exhibited the best results, with inhibition rates of 50.2% against , 36.5% against , 47.5% against , 31.8% against , and 24.7% against . There were no significant differences in the growth of adipose mesenchymal stem cells between the prepared samples and the control. For osteogenic differentiation, dye uptake was 1.2 times higher for HA and 5 wt% Zn-HA, and 1.3 times higher for 3 wt% Zn-HA compared to the control. These results suggest that developed ceramics may be effective in regenerative medicine, paving the way for innovative treatments.
细菌耐药性以及对新型抗菌策略的需求是当代医学面临的主要挑战。在本研究中，采用湿沉淀法合成了锌含量分别为3 wt%、5 wt%和10 wt%的锌掺杂羟基磷灰石（Zn-HA）样品，并在700℃和800℃下烧结。通过X射线衍射、傅里叶变换红外光谱、拉曼光谱和扫描电子显微镜对样品进行了表征。测试了Zn-HA对四种细菌菌株——、、、以及白色念珠菌的抗菌性能。5 wt%和10 wt%的Zn-HA均能有效抑制所有微生物的生长。值得注意的是，10 wt%的Zn-HA表现出最佳效果，对的抑制率为50.2%，对的抑制率为36.5%，对的抑制率为47.5%，对的抑制率为31.8%，对的抑制率为24.7%。制备的样品与对照之间脂肪间充质干细胞的生长没有显著差异。对于成骨分化，与对照相比，HA和5 wt% Zn-HA的染料摄取量高1.2倍，3 wt% Zn-HA的染料摄取量高1.3倍。这些结果表明，所开发的陶瓷在再生医学中可能有效，为创新治疗铺平了道路。
