五肽重复蛋白OsPPR674通过调节线粒体转录本的RNA编辑来调控水稻生长和干旱敏感性。

The Pentatricopeptide Repeat Protein OsPPR674 Regulates Rice Growth and Drought Sensitivity by Modulating RNA Editing of the Mitochondrial Transcript .

作者信息

Li Jinglei, Zhang Longhui, Li Chenyang, Chen Weijun, Wang Tiankang, Tan Lvni, Qiu Yingxin, Song Shufeng, Li Bin, Li Li

机构信息

Longping Branch, College of Biology, Hunan University, Changsha 410125, China.

State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Hunan Academy of Agricultural Sciences, Changsha 410125, China.

出版信息

Int J Mol Sci. 2025 Mar 14;26(6):2646. doi: 10.3390/ijms26062646.

The P-type pentatricopeptide repeat (PPR) proteins are crucial for RNA editing and post-transcriptional regulation in plant organelles, particularly mitochondria. This study investigates the role of in rice, focusing on its function in mitochondrial RNA editing. Using CRISPR/Cas9 technology, we generated mutant and examined its phenotypic and molecular characteristics. The results indicate that exhibits reduced plant height, decreased seed-setting rate, and poor drought tolerance. Further analysis revealed that in the mutant, RNA editing at the 299th nucleotide position of the mitochondrial gene (C-to-U conversion) was abolished. REMSAs showed that GST- specifically binds to RNA probes targeting this -299 site, confirming its role in this editing process. In summary, these results suggest that plays a pivotal role in mitochondrial RNA editing, emphasizing the significance of PPR proteins in organelle function and plant development.

P型五肽重复（PPR）蛋白对于植物细胞器尤其是线粒体中的RNA编辑和转录后调控至关重要。本研究调查了其在水稻中的作用，重点关注其在线粒体RNA编辑中的功能。利用CRISPR/Cas9技术，我们生成了突变体并检测了其表型和分子特征。结果表明，该突变体株高降低、结实率下降且耐旱性差。进一步分析显示，在该突变体中，线粒体基因第299个核苷酸位置的RNA编辑（C到U的转换）被消除。RNA电泳迁移率变动分析表明，谷胱甘肽S-转移酶（GST）特异性结合靶向该-299位点的RNA探针，证实了其在该编辑过程中的作用。总之，这些结果表明在水稻线粒体RNA编辑中起关键作用，强调了PPR蛋白在细胞器功能和植物发育中的重要性。