Medić Alan, Savić Vladimir, Klobučar Ana, Bogdanić Maja, Curman Posavec Marcela, Nonković Diana, Barbić Ljubo, Rončević Ivana, Stevanović Vladimir, Vilibić-Čavlek Tatjana
Department of Epidemiology, Zadar County Institute of Public Health, 23000 Zadar, Croatia.
Department of Health Studies, University of Zadar, 23000 Zadar, Croatia.
Microorganisms. 2025 Mar 2;13(3):565. doi: 10.3390/microorganisms13030565.
Autochthonous dengue cases have been continuously recorded in Europe in the past two decades. The first autochthonous dengue case in Croatia was reported in 2010 on the Pelješac Peninsula, while imported cases were recorded continuously thereafter. In 2024, dengue re-emerged in Croatia. An epidemiological and entomological study was conducted after receiving information on dengue virus (DENV) infection in a German tourist probably acquired on Dugi Otok Island in Croatia in May 2024. Serum samples were collected from 30 residents of the Veli Rat region where the patient had stayed. In addition, mosquitoes were collected in the same area. Human samples were tested for the presence of DENV antibodies (ELISA and IFA) and DENV RNA (RT-qPCR), while mosquito samples were tested for DENV RNA (RT-qPCR). DENV IgM or IgG antibodies were found in 8 serum samples, while no one sample was RT-qPCR positive. No cross-reactivity with flaviviruses was detected in seropositive samples, supporting DENV infection. One patient was classified as a confirmed dengue case (IgG seroconversion in paired serum samples) and five as probable cases (IgM detection in a single serum sample). One additional patient, sampled only once, was IgG seropositive. Two of the seropositive individuals reported fever and rash three weeks before testing. The re-emergence of dengue in Croatia highlights the need for continuous monitoring of DENV circulation in both humans and vectors.
在过去二十年中，欧洲持续记录到本土登革热病例。克罗地亚的首例本土登革热病例于2010年在佩列沙茨半岛被报告，此后不断有输入性病例被记录。2024年，登革热在克罗地亚再次出现。在收到关于一名德国游客可能于2024年5月在克罗地亚的杜吉奥托克岛感染登革热病毒（DENV）的信息后，开展了一项流行病学和昆虫学研究。从患者停留过的韦利拉特地区的30名居民中采集了血清样本。此外，在同一地区收集了蚊子样本。对人类样本进行了DENV抗体（ELISA和IFA）和DENV RNA（RT-qPCR）检测，而对蚊子样本进行了DENV RNA（RT-qPCR）检测。在8份血清样本中发现了DENV IgM或IgG抗体，但没有一份样本RT-qPCR呈阳性。在血清阳性样本中未检测到与黄病毒的交叉反应，支持DENV感染。一名患者被分类为确诊登革热病例（配对血清样本中IgG血清转化），五名患者被分类为可能病例（单个血清样本中检测到IgM）。另有一名仅采样一次的患者IgG血清呈阳性。两名血清阳性个体报告在检测前三周出现发热和皮疹。克罗地亚登革热的再次出现凸显了对人类和病媒中DENV传播进行持续监测的必要性。
