: Phytochemistry, Antimicrobial Potential with Antibiotic Enhancement, and Toxicity Insights.

Linn. (commonly known as Amla or Indian Gooseberry) is commonly used in Ayurvedic medicine to treat respiratory infections, skin disorders, and gastrointestinal issues. The fruit contains an abundance of polyphenols, which contribute to its strong antioxidant properties. The antibacterial activity of fruit extracts derived from against , , and was determined along with the antibiotic-resistant variants extended-spectrum β-lactamase (ESBL) , methicillin-resistant (MRSA), and ESBL . Disc diffusion and broth dilution assays were conducted to assess the activity of aqueous, methanolic, and ethyl acetate extracts, with large zones of inhibition of up to 15 mm on agar observed for and MRSA. Minimum inhibitory concentration (MIC) values ranging from 158 to 1725 µg/mL were calculated. The aqueous and methanolic extracts of were less active against , ESBL , , and , with the only noteworthy MIC (633 µg/mL) observed for the aqueous extract against . Interestingly, a lack of inhibition was observed on agar for any of the extracts against these bacteria. Liquid chromatography-mass spectrometry (LC-MS) analysis identified several notable flavonoids, phenolic acids, terpenoids, and tannins. Notably, bioassays indicated that all extracts were nontoxic. The antibacterial activity and absence of toxicity in extracts suggest their potential as candidates for antibiotic development, highlighting the need for further mechanistic and phytochemical investigations.