Nam Sohee, Kim Yujin, Lee Min Ji, Kim Yeon Bee, Yun Jeong Ui, Jung Mi-Ja, Song Hye Seon, Lee Se Hee, Kim Seok-Jun, Whon Tae Woong
Kimchi Functionality Research Group, World Institute of Kimchi, Gwangju, Republic of Korea.
Department of Integrative Biological Sciences, Chosun University, Gwangju, Republic of Korea.
Int J Syst Evol Microbiol. 2025 Mar;75(3). doi: 10.1099/ijsem.0.006722.
A novel Gram-stain-positive, non-spore-forming, non-motile and rod-shaped bacterium, designated strain CBA3109, was isolated from jogae-jeotgal (fermented clam), a traditional Korean fermented seafood. Strain CBA3109 showed growth at 10-30 °C (optimum, 25 °C) and pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-15% (w/v) NaCl (optimum, 5%). Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain CBA3109 belonged to the genus , with the highest similarities to NCDO 739 (98.26%) and VKM Ac-2118 (98.14%). Strain CBA3109 contained MK-8(H) as the major menaquinone. The cell wall peptidoglycan contained -diaminopimelic acid. The major fatty acids (>10%) were anteiso-C and anteiso-C. The main polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The average nucleotide identity and digital DNA-DNA hybridization values between strain CBA3109 and the closest species were 86.2-87.0% and 31.1-33.0 %, respectively. The DNA G+C content of strain CBA3109 was 62.7%. Based on the morphological, phylogenetic, chemotaxonomic and genotypic data, strain CBA3109 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CBA3109 (= KACC 23387 = DSM 117564).
从韩国传统发酵海鲜腌渍蛤中分离出一株新型革兰氏阳性、无芽孢、不运动的杆状细菌，命名为CBA3109菌株。CBA3109菌株在10-30°C（最适温度为25°C）、pH值6.0-9.0（最适pH值为7.0）以及0-15%（w/v）NaCl（最适浓度为5%）的条件下能够生长。对16S rRNA基因序列进行系统发育分析表明，CBA3109菌株属于[具体属名未给出]属，与NCDO 739（98.26%）和VKM Ac-2118（98.14%）的相似度最高。CBA3109菌株以MK-8(H)作为主要甲基萘醌。细胞壁肽聚糖含有[具体名称未给出]-二氨基庚二酸。主要脂肪酸（>10%）为anteiso-C和anteiso-C。主要极性脂为双磷脂酰甘油和磷脂酰甘油。CBA3109菌株与最接近物种之间的平均核苷酸同一性和数字DNA-DNA杂交值分别为86.2-87.0%和31.1-33.0%。CBA3109菌株的DNA G+C含量为62.7%。基于形态学、系统发育学、化学分类学和基因型数据，CBA3109菌株代表了[具体属名未给出]属的一个新物种，为此提出新物种名称[具体名称未给出] sp. nov.。模式菌株为CBA3109（= KACC 23387 = DSM 117564）。
