Miyai Hisataka, Tomofuji Takaaki, Mizuno Hirofumi, Morita Manabu, Nakahara Momoko, Kataoka Kota, Sumita Ichiro, Uchida Yurika, Toyama Naoki, Yokoi Aya, Yamanaka-Kohno Reiko, Takeuchi Noriko, Maruyama Takayuki, Ekuni Daisuke
Department of Preventive Dentistry, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8558, Japan.
Shiotsu Dental Clinic, Kurashiki 710-0142, Japan.
Healthcare (Basel). 2025 Mar 13;13(6):619. doi: 10.3390/healthcare13060619.
Halitosis is a condition characterized by an unpleasant malodor. Intra-oral halitosis is caused by volatile sulfur compounds (VSCs) and can be associated with oral dryness. Trehalose is one of the materials used to relieve oral dryness. The aim of the present study was to investigate the effect of trehalose on halitosis. This prospective, double-blinded, placebo-controlled, cross-over study enrolled volunteers from Okayama University Hospital. The participants were randomly divided into two groups, with one group receiving trehalose (a 10% trehalose solution) and the other receiving a placebo (distilled water) in a 1:1 allocation. The primary study outcome was the subjective organoleptic test. The secondary outcomes were the concentrations of the VSCs, which were measured using a portable gas chromatography device, and the oral moisture status, which was measured using an oral moisture meter. The planned sample size was 10 participants based on the previous study. The final intention-to-treat analysis was performed using the data from 9 participants. After applying 10% trehalose as an oral spray, the organoleptic score decreased in a time-dependent manner. However, no significant differences were seen between the trehalose and placebo groups. In terms of secondary outcomes, the oral moisture levels increased immediately after the trehalose spray application, and significant differences in the amount of change from the baseline were seen between the trehalose and placebo groups ( = 0.047). No significant differences were seen in any of the other variables ( > 0.05). We could not identify any positive effects on halitosis from a one-time 10% trehalose application as an oral spray in this prospective, double-blinded, placebo-controlled, cross-over study. However, the trehalose application immediately improved the oral moisture levels and was useful for treating oral dryness.
口臭是一种以难闻气味为特征的病症。口腔内口臭由挥发性硫化物（VSCs）引起，且可能与口腔干燥有关。海藻糖是用于缓解口腔干燥的物质之一。本研究的目的是调查海藻糖对口臭的影响。这项前瞻性、双盲、安慰剂对照、交叉研究招募了来自冈山大学医院的志愿者。参与者被随机分为两组，一组接受海藻糖（10%海藻糖溶液），另一组按1:1分配接受安慰剂（蒸馏水）。主要研究结果是主观感官测试。次要结果是使用便携式气相色谱仪测量的VSCs浓度以及使用口腔湿度计测量的口腔水分状况。根据先前的研究，计划样本量为10名参与者。最终的意向性分析使用了9名参与者的数据。以口腔喷雾形式应用10%海藻糖后，感官评分呈时间依赖性下降。然而，海藻糖组和安慰剂组之间未见显著差异。在次要结果方面，海藻糖喷雾应用后口腔水分水平立即升高，海藻糖组和安慰剂组从基线开始的变化量存在显著差异（P = 0.047）。在任何其他变量中均未见显著差异（P > 0.05）。在这项前瞻性、双盲、安慰剂对照、交叉研究中，我们未发现一次性以口腔喷雾形式应用10%海藻糖对口臭有任何积极影响。然而，海藻糖的应用立即改善了口腔水分水平，对治疗口腔干燥有用。
