N Mowat Ashwaq, Al-Abady Zainab N
Department of Chemistry, Faculty of Sciences, University of Al-Qadisiyah, Iraq.
Asian Pac J Cancer Prev. 2025 Mar 1;26(3):877-887. doi: 10.31557/APJCP.2025.26.3.877.
Colon cancer is a major health concern globally, being one of the most commonly diagnosed cancers. Inflammation and oxidative stress are critical factors contributing to its development and progression. Understanding the roles of key molecules such as NOX1, TNF-α, and COX-2 in these processes may provide insights into potential biomarkers for early detection and targeted therapy.
This study aims to investigate the roles of NADPH oxidase 1 (NOX1), Tumor Necrosis Factor-alpha (TNF-α), and Cyclooxygenase-2 (COX-2) in colon cancer progression and their possible implications in the disease context.
A comparative analysis was conducted involving 40 colon cancer patients (15 males, 25 females; aged 20-78 years) and 40 healthy controls (17 males, 23 females; aged 21-75 years). Blood samples were collected from participants between October 10, 2023, and February 18, 2024. The levels of NOX1, TNF-α, and COX-2 were measured using the enzyme-linked immunosorbent assay (ELISA) method.
The study found significantly elevated levels of TNF-α in colon cancer patients (100.9-454.3 ng/L) compared to healthy controls (24.85-216.9 ng/L). Additionally, COX-2 levels were markedly higher in patients (239.4-690.53 units/L) than in controls (23.78-115.5 units/L). NOX1 levels were also elevated in cancer patients (4.0-14.92) relative to healthy subjects (0.89-9.39). These findings suggest an association between increased levels of TNF-α, COX-2, and NOX1 with a higher risk of colon cancer.
The findings suggest that elevated levels of TNF-α, COX-2, and NOX1 may be associated with the progression of colon cancer, indicating their potential involvement in the disease. Specifically, higher levels of TNF-α and COX-2 could be linked to increased cancer cell proliferation and metastasis, while elevated NOX1 levels might be related to oxidative stress and cellular transformation in colon cancer patients.
结肠癌是全球主要的健康问题之一，是最常被诊断出的癌症之一。炎症和氧化应激是其发生和发展的关键因素。了解诸如NOX1、肿瘤坏死因子-α（TNF-α）和环氧化酶-2（COX-2）等关键分子在这些过程中的作用，可能为早期检测和靶向治疗的潜在生物标志物提供见解。
本研究旨在探讨烟酰胺腺嘌呤二核苷酸磷酸氧化酶1（NOX1）、肿瘤坏死因子-α（TNF-α）和环氧化酶-2（COX-2）在结肠癌进展中的作用及其在疾病背景下的可能影响。
对40例结肠癌患者（15例男性，25例女性；年龄20 - 78岁）和40例健康对照者（17例男性，23例女性；年龄21 - 75岁）进行了比较分析。于2023年10月10日至2024年2月18日期间采集参与者的血液样本。采用酶联免疫吸附测定（ELISA）法检测NOX1、TNF-α和COX-2的水平。
研究发现，与健康对照者（24.85 - 216.9 ng/L）相比，结肠癌患者的TNF-α水平显著升高（100.9 - 454.3 ng/L）。此外，患者的COX-2水平（239.4 - 690.53单位/L）明显高于对照者（23.78 - 115.5单位/L）。与健康受试者（0.89 - 9.39）相比，癌症患者的NOX1水平也有所升高（4.0 - 14.92）。这些发现表明TNF-α、COX-2和NOX1水平升高与结肠癌风险增加之间存在关联。
研究结果表明，TNF-α、COX-2和NOX1水平升高可能与结肠癌的进展有关，表明它们可能参与了该疾病。具体而言，较高水平的TNF-α和COX-2可能与癌细胞增殖和转移增加有关，而NOX1水平升高可能与结肠癌患者的氧化应激和细胞转化有关。
