Manwaring J D, Csallany A S
J Nutr. 1981 Dec;111(12):2172-9. doi: 10.1093/jn/111.12.2172.
A systematic search was conducted to find fluorescent compounds in water-soluble mouse tissue extracts which may be related to dietary vitamin E. Female weanling mice were fed a vitamin E deficient or a vitamin E supplemented diet for 14 months. The lung, liver, spleen, kidney, heart, and brain were homogenized and extracted with 2:1 chloroform:methanol and water. The water was lyophilized and the fluorescent compounds were separated by Sephadex G-15 or G-25 column chromatography. For each tissue, thorough excitation and emission spectra were determined for the eluting fractions, then the fluorescence of each fraction was measured at the resulting excitation and emission wavelength maxima. Up to four fluorescent water-soluble compounds responded to dietary vitamin E, depending on the tissue. Vitamin E in the diet led to the decreased accumulation of three of the fluorescent compounds, with excitation and emission wavelengths of: 275 nm/350 nm, 270 nm/310 nm, and 275 nm/350 nm. The fourth compound (320 nm/380 nm) is probably protected by vitamin E in the diet.
进行了一项系统搜索，以寻找水溶性小鼠组织提取物中可能与膳食维生素E相关的荧光化合物。将雌性断乳小鼠喂食维生素E缺乏或补充维生素E的饮食14个月。将肺、肝、脾、肾、心脏和脑匀浆，并用2:1的氯仿：甲醇和水进行提取。将水冻干，并用葡聚糖G - 15或G - 25柱色谱法分离荧光化合物。对于每个组织，测定洗脱级分的完整激发光谱和发射光谱，然后在所得的激发和发射波长最大值处测量每个级分的荧光。根据组织不同，多达四种水溶性荧光化合物对膳食维生素E有反应。饮食中的维生素E导致三种荧光化合物的积累减少，其激发和发射波长分别为：275 nm/350 nm、270 nm/310 nm和275 nm/350 nm。第四种化合物（320 nm/380 nm）可能受到饮食中维生素E的保护。
