ShangGuan Yuwen, Huang Kunyi, Zhu Zining, Yuan Yuan, Song Yawei, Wang Hao, Chen Liang, Yu Shiqi, Zheng Guangzhi, Liang Qi
Institute of Competitive Sports, Nanjing Sport Institute, Nanjing, China.
Department of Exercise Physiology, Kunsan National University, Gunsan, Republic of Korea.
Front Physiol. 2025 Mar 17;16:1508305. doi: 10.3389/fphys.2025.1508305. eCollection 2025.
This study aims to investigate the effects of low-intensity blood flow restriction training on myocardial tissue in rats. By measuring the levels of myocardial injury biomarkers in serum and the expression of anti-apoptotic and antioxidant proteins in myocardial tissue, the study preliminarily explores the underlying mechanisms.
Male 3-month-old Sprague-Dawley rats were randomly divided into the following groups: control group (CON), low-intensity training group (LIRT), high-intensity training group (HIRT), and low-intensity blood flow restriction training group (LIBFR), with 6 rats in each group. Body weight, maximum voluntary carrying capacity, myocardial morphology, myocardial injury biomarkers, and the expression levels of Bcl-2, Bax, Nrf2, and Keap1 proteins in myocardial tissue were evaluated.
(1)cTn1 Detection: The HIRT group showed a significant increase in cTn1 levels (P < 0.01), while the LIBFR group had a lower cTn1 level compared to the HIRT group (P < 0.05). (2)Nrf2 and Keap1 Results: Compared to the CON group, the LIBFR group showed an increase in Nrf2 (P < 0.05), and a significant increase in Keap1 (P < 0.01). (3)Bcl-2 and Bax Results: Compared to the CON group, Bcl-2 levels were significantly elevated in the HIRT group (P < 0.01) and increased in the LIBFR group (P < 0.05), while Bax expression was significantly reduced in the LIBFR group (P < 0.05). Regarding the Bcl-2/Bax ratio, the LIRT, HIRT, and LIBFR groups exhibited significantly higher values compared to the CON group (P < 0.01). Furthermore, the HIRT and LIBFR groups showed significantly higher Bcl-2/Bax ratios than the LIRT group (P < 0.01).
Low-intensity blood flow restriction training can effectively reduce cTn1 in rat serum, decrease cardiomyocyte apoptosis, and improve antioxidant capacity, which has a certain protective effect on the myocardium.
本研究旨在探讨低强度血流限制训练对大鼠心肌组织的影响。通过检测血清中心肌损伤生物标志物的水平以及心肌组织中抗凋亡和抗氧化蛋白的表达，本研究初步探究其潜在机制。
将3个月大的雄性Sprague-Dawley大鼠随机分为以下几组：对照组（CON）、低强度训练组（LIRT）、高强度训练组（HIRT）和低强度血流限制训练组（LIBFR），每组6只大鼠。评估体重、最大自愿承载能力、心肌形态、心肌损伤生物标志物以及心肌组织中Bcl-2、Bax、Nrf2和Keap1蛋白的表达水平。
（1）肌钙蛋白I（cTn1）检测：高强度训练组cTn1水平显著升高（P<0.01），而低强度血流限制训练组的cTn1水平低于高强度训练组（P<0.05）。（2）Nrf2和Keap1结果：与对照组相比，低强度血流限制训练组Nrf2增加（P<0.05），Keap1显著增加（P<0.01）。（3）Bcl-2和Bax结果：与对照组相比，高强度训练组Bcl-2水平显著升高（P<0.01），低强度血流限制训练组Bcl-2水平升高（P<0.05），而低强度血流限制训练组Bax表达显著降低（P<0.05）。关于Bcl-2/Bax比值，低强度训练组、高强度训练组和低强度血流限制训练组的值均显著高于对照组（P<0.01）。此外，高强度训练组和低强度血流限制训练组的Bcl-2/Bax比值显著高于低强度训练组（P<0.01）。
低强度血流限制训练可有效降低大鼠血清中的cTn1，减少心肌细胞凋亡，提高抗氧化能力，对心肌具有一定的保护作用。
