温和型枯草芽孢杆菌噬菌体phi 3T：染色体附着位点及与温和型噬菌体phi 105和SPO2的比较
Temperate Bacillus subtilis bacteriophage phi 3T: chromosomal attachment site and comparison with temperate bacteriophages phi 105 and SPO2.
作者信息
Williams M T, Young F E
出版信息
J Virol. 1977 Feb;21(2):522-9. doi: 10.1128/JVI.21.2.522-529.1977.
Abstract
The temperate Bacillus subtilis bacteriophage phi 3T contains within its genome a locus, designated thyP3, that encodes for a protein with thymidylate synthetase activity. Bacteriophage phi 3T is different from the two previously characterized temperate phages, phi 105 and SPO2, in: heteroimmunity, response to bacteriophage antisera, endonuclease digestion pattern, induction in the presence of 6-(p-hydroxyphenylazo)-uracil, and effect on the lytic cycle of bacteriophage phi 1. The mean burst size of phi 3T is 56. The dose response curve with bacteriophage phi 3T DNA is linear for transfection and transformation to the Thy+ phenotype. The inserted prophage has been mapped by PBS1 transduction; it is between chromosomal markers ilvA8 and gltA in the terminus of the chromosome. Thus thyP3 maps at a site separate from, but between, the bacterial markers thyA and thyB when thyP3 is in the prophage state.
摘要
温和型枯草芽孢杆菌噬菌体 phi 3T 在其基因组中含有一个名为 thyP3 的位点，该位点编码一种具有胸苷酸合成酶活性的蛋白质。噬菌体 phi 3T 与之前鉴定的两种温和型噬菌体 phi 105 和 SPO2 在以下方面存在差异：异源性免疫、对噬菌体抗血清的反应、核酸内切酶消化模式、在 6-(对羟基苯偶氮)-尿嘧啶存在下的诱导作用以及对噬菌体 phi 1 裂解周期的影响。phi 3T 的平均裂解量为 56。噬菌体 phi 3T DNA 的剂量反应曲线在转染和转化为 Thy+表型时呈线性。插入的原噬菌体已通过 PBS1 转导进行定位；它位于染色体末端的染色体标记 ilvA8 和 gltA 之间。因此，当 thyP3 处于原噬菌体状态时，它定位于与细菌标记 thyA 和 thyB 分开但位于它们之间的位点。
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