Achilli Ana Laura, Kumar Naveen, Fetterley Vincent, Khangura Rajdeep S, Brar Gurcharn Singh
Faculty of Land and Food Systems, The University of British Columbia, Vancouver, BC, Canada.
Faculty of Agricultural, Life and Environmental Science, University of Alberta, Edmonton, AB, Canada.
Methods Mol Biol. 2025;2898:261-272. doi: 10.1007/978-1-0716-4378-5_16.
The three wheat rusts, stripe rust (caused by Puccinia striiformis f. sp. tritici), leaf rust (caused by Puccinia triticina), and stem rust (caused by Puccinia graminis f. sp. tritici), are among the most important wheat diseases causing significant yield losses worldwide. Improving rust resistance in wheat cultivars is an effective way to maintain yield under rust epidemic conditions. The identification of novel rust resistance quantitative trait loci (QTLs) along with the development of genetic markers associated with these QTLs may facilitate the development of rust resistance in wheat cultivars. Therefore, this chapter summarizes the protocols and methodologies for statistical analysis of rust resistance in segregating populations at seedling and adult plant stages to identify QTL that confer rust resistance in wheat. QTL mapping is also a prerequisite for fine mapping of the genomic regions to identify an underlying genetic cause. For breeding, molecular markers that perfectly co-segregate with a linked genetic variant facilitates marker-assisted selection.
三种小麦锈病，即条锈病（由条形柄锈菌小麦专化型引起）、叶锈病（由小麦叶锈菌引起）和秆锈病（由禾柄锈菌小麦专化型引起），是造成全球小麦显著减产的最重要的小麦病害。提高小麦品种的锈病抗性是在锈病流行条件下维持产量的有效途径。鉴定新的抗锈病数量性状位点（QTL）以及开发与这些QTL相关的遗传标记，可能有助于培育抗锈病小麦品种。因此，本章总结了在分离群体的幼苗期和成年植株期进行抗锈性统计分析以鉴定赋予小麦抗锈性的QTL的方案和方法。QTL定位也是对基因组区域进行精细定位以确定潜在遗传原因的前提条件。对于育种而言，与连锁遗传变异完全共分离的分子标记有助于标记辅助选择。
