Sharma Jyoti Saini, Singh Lovepreet
Department of Plant Pathology, University of Minnesota, St. Paul, MN, USA.
Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN, USA.
Methods Mol Biol. 2025;2898:273-280. doi: 10.1007/978-1-0716-4378-5_17.
The marker-assisted breeding (MAB) approach has become more convenient and time efficient with the advancement of allele-specific, cost-effective, and breeder-friendly single nucleotide polymorphism (SNP)-based kompetitive allele-specific (KASP) polymerase chain reaction (PCR) markers. Here, we describe a detailed protocol to develop KASP assays for rust resistance breeding in wheat.
随着基于等位基因特异性、经济高效且对育种者友好的单核苷酸多态性（SNP）的竞争性等位基因特异性（KASP）聚合酶链反应（PCR）标记的发展，标记辅助育种（MAB）方法变得更加便捷且节省时间。在此，我们描述了一种用于开发小麦抗锈病育种KASP检测方法的详细方案。
