Singh Lovepreet, Anderson James A, Chen Jianli, Gill Bikram S, Tiwari Vijay K, Rawat Nidhi
Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD 20742, USA.
Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN 55108, USA.
Plant Pathol J. 2019 Jun;35(3):200-207. doi: 10.5423/PPJ.OA.01.2019.0018. Epub 2019 Jun 1.
Fusarium head blight (FHB) is a devastating wheat disease with a significant economic impact. is the most important large effect and stable QTL for FHB resistance. A pore-forming toxin-like () gene was recently identified as an underlying gene for resistance. In this study, we developed and validated a -based Kompetitive allele specific PCR (KASP) marker for . The KASP marker, PFT_KASP, was used to screen 298 diverse wheat breeding lines and cultivars. The KASP clustering results were compared with gel-based gene specific markers and the widely used linked STS marker, UMN10. Eight disagreements were found between PFT_KASP and UMN10 assays among the tested lines. Based on the genotyping and sequencing of genes in the region, these genotypes were found to be common with a previously characterized susceptible haplotype. Therefore, our results indicate that PFT_KASP is a perfect diagnostic marker for and would be a valuable tool for introgression and pyramiding of FHB resistance in wheat cultivars.
赤霉病（FHB）是一种对小麦具有毁灭性影响且造成重大经济损失的病害。[具体基因名称]是赤霉病抗性最重要的主效且稳定的数量性状基因座（QTL）。最近，一个类成孔毒素（[具体基因名称]）基因被鉴定为[具体基因名称]抗性的潜在基因。在本研究中，我们开发并验证了一种基于[具体基因名称]的竞争性等位基因特异性PCR（KASP）标记。该KASP标记PFT_KASP用于筛选298个不同的小麦育种系和品种。将KASP聚类结果与基于凝胶的基因特异性标记以及广泛使用的连锁STS标记UMN10进行了比较。在测试的品系中，发现PFT_KASP和UMN10检测之间存在8个不一致之处。基于[具体基因区域]基因的基因分型和测序，发现这些基因型与先前鉴定的易感单倍型相同。因此，我们的结果表明PFT_KASP是[具体基因名称]的完美诊断标记，将成为小麦品种中赤霉病抗性渗入和聚合的有价值工具。
