一种在优良小麦品种中进行高效转化和基因组编辑的方案。
A Protocol for High-efficiency Transformation and Genome Editing in Elite Wheat Cultivars.
作者信息
Kaur Karminderbir, Biswal Akshaya K, Kaur Rajvinder, Singh Mehtab-, Dhugga Kanwarpal S, Singh Jaswinder
机构信息
Department of Plant Science, McGill University, Ste Anne de Bellevue, QC, Canada.
International Maize and Wheat Improvement Center (CIMMYT), Ciudad dee México, Mexico.
出版信息
Methods Mol Biol. 2025;2898:307-320. doi: 10.1007/978-1-0716-4378-5_20.
CRISPR-Cas9-based editing offers a simple yet efficient method for introducing targeted mutations in the genes of interest. Low efficiency of transformation and a lack of reproducibility in editing genes in elite wheat cultivars are the bottlenecks in improving simply inherited traits. We describe a high-efficiency transformation protocol for editing elite wheat cultivars using JD633-GRF4-GIF1 CRISPR vector. This vector reduces the regeneration time of transformants to less than 90 days and enables transformation of elite cultivars.
基于CRISPR-Cas9的编辑为在目标基因中引入靶向突变提供了一种简单而有效的方法。在优良小麦品种中，转化效率低和基因编辑缺乏可重复性是改良简单遗传性状的瓶颈。我们描述了一种使用JD633-GRF4-GIF1 CRISPR载体编辑优良小麦品种的高效转化方案。该载体将转化体的再生时间缩短至90天以内，并能够对优良品种进行转化。
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