基因组编辑：过去、现在与未来。
Genome Editing: Past, Present, and Future.
作者信息
Carroll Dana
机构信息
Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT.
出版信息
Yale J Biol Med. 2017 Dec 19;90(4):653-659. eCollection 2017 Dec.
Abstract
The CRISPR-Cas genome editing tools have been adopted rapidly in the research community, and they are quickly finding applications in the commercial sector as well. Lest we lose track of the broader context, this Perspective presents a brief review of the history of the genome editing platforms and considers a few current technological issues. It then takes a very limited view into the future of this technology and highlights some of the societal issues that require examination and discussion.
摘要
CRISPR-Cas基因组编辑工具已在研究领域迅速得到应用，并且它们也很快在商业领域找到用武之地。为免我们忽略更广泛的背景，本视角文章简要回顾了基因组编辑平台的历史，并思考了当前的一些技术问题。然后，它对这项技术的未来进行了非常有限的展望，并强调了一些需要审视和讨论的社会问题。
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本文引用的文献
1
Genome editing reveals a role for OCT4 in human embryogenesis.
Nature. 2017 Oct 5;550(7674):67-73. doi: 10.1038/nature24033. Epub 2017 Sep 20.
2
Correction of a pathogenic gene mutation in human embryos.
Nature. 2017 Aug 24;548(7668):413-419. doi: 10.1038/nature23305. Epub 2017 Aug 2.
3
Simultaneous modification of three homoeologs of TaEDR1 by genome editing enhances powdery mildew resistance in wheat.
Plant J. 2017 Aug;91(4):714-724. doi: 10.1111/tpj.13599. Epub 2017 Jun 13.
4
Synthetically modified guide RNA and donor DNA are a versatile platform for CRISPR-Cas9 engineering.
Elife. 2017 May 2;6:e25312. doi: 10.7554/eLife.25312.
5
Precision genome editing using CRISPR-Cas9 and linear repair templates in C. elegans.
Methods. 2017 May 15;121-122:86-93. doi: 10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7.
6
Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells.
Nat Methods. 2016 Dec;13(12):1036-1042. doi: 10.1038/nmeth.4038. Epub 2016 Oct 31.
7
Direct stacking of sequence-specific nuclease-induced mutations to produce high oleic and low linolenic soybean oil.
BMC Plant Biol. 2016 Oct 13;16(1):225. doi: 10.1186/s12870-016-0906-1.
8
Targeted AID-mediated mutagenesis (TAM) enables efficient genomic diversification in mammalian cells.
Nat Methods. 2016 Dec;13(12):1029-1035. doi: 10.1038/nmeth.4027. Epub 2016 Oct 10.
9
Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity.
Mol Cell. 2016 Aug 4;63(3):355-70. doi: 10.1016/j.molcel.2016.07.004.
10
Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems.
Science. 2016 Sep 16;353(6305). doi: 10.1126/science.aaf8729. Epub 2016 Aug 4.
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