基因组编辑：过去、现在与未来。
Genome Editing: Past, Present, and Future.
作者信息
Carroll Dana
机构信息
Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT.
出版信息
Yale J Biol Med. 2017 Dec 19;90(4):653-659. eCollection 2017 Dec.
Abstract
The CRISPR-Cas genome editing tools have been adopted rapidly in the research community, and they are quickly finding applications in the commercial sector as well. Lest we lose track of the broader context, this Perspective presents a brief review of the history of the genome editing platforms and considers a few current technological issues. It then takes a very limited view into the future of this technology and highlights some of the societal issues that require examination and discussion.
摘要
CRISPR-Cas基因组编辑工具已在研究领域迅速得到应用，并且它们也很快在商业领域找到用武之地。为免我们忽略更广泛的背景，本视角文章简要回顾了基因组编辑平台的历史，并思考了当前的一些技术问题。然后，它对这项技术的未来进行了非常有限的展望，并强调了一些需要审视和讨论的社会问题。
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2
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Nature. 2017 Aug 24;548(7668):413-419. doi: 10.1038/nature23305. Epub 2017 Aug 2.
3
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Plant J. 2017 Aug;91(4):714-724. doi: 10.1111/tpj.13599. Epub 2017 Jun 13.
4
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Elife. 2017 May 2;6:e25312. doi: 10.7554/eLife.25312.
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Methods. 2017 May 15;121-122:86-93. doi: 10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7.
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