Dennis P J, Lee J V
Quality Control and Safety Laboratory, Service Centre for Applied Microbiology and Research, Salisbury, Wilts, UK.
J Appl Bacteriol. 1988 Aug;65(2):135-41. doi: 10.1111/j.1365-2672.1988.tb01501.x.
A strain of Legionella pneumophila serogroup 1 known to be virulent for guinea-pigs was found to be least stable at a relative humidity (r.h.) of 60% when stored as a small particle aerosol. Three L. pneumophila serogroup 1 strains of different virulence for guinea-pigs were then tested at a r.h. of 60% at 20 degrees C. The most virulent strain was found to have the best survival and the avirulent strain was least stable. The strain of intermediate virulence did not survive as well as the virulent strain but was more stable than the avirulent strain. Strains of L. pneumophila serogroup epidemiologically associated with legionnaires' disease had better survival in small particle aerosols than strains which were not associated with disease. Subtyping with monoclonal antibodies also showed that the type more commonly associated with disease survived longer in aerosols than the other subtypes.
一种已知对豚鼠具有致病性的嗜肺军团菌血清1型菌株，当以小颗粒气溶胶形式储存时，在相对湿度（r.h.）为60%时稳定性最差。随后，对三种对豚鼠具有不同毒力的嗜肺军团菌血清1型菌株在20摄氏度、相对湿度60%的条件下进行了测试。结果发现，毒力最强的菌株存活能力最佳，无毒力菌株稳定性最差。中等毒力菌株的存活情况不如毒力强的菌株，但比无毒力菌株更稳定。在流行病学上与军团病相关的嗜肺军团菌血清型菌株，在小颗粒气溶胶中的存活能力优于与疾病无关的菌株。用单克隆抗体进行亚型分析还表明，更常见于疾病相关的类型在气溶胶中的存活时间比其他亚型更长。
