Pesqueira Ana M, González Ana M, Barragán-Lozano Teresa, Arnedo María S, Lozano Rafael, Santalla Marta
Grupo de Genética del Desarrollo de Plantas, Misión Biológica de Galicia-CSIC, P.O. Box 28, 36080 Pontevedra, Spain.
Centro de Investigación en Biotecnología Agroalimentaria (CIAIMBITAL), Universidad de Almería, 04120 Almería, Spain.
Plants (Basel). 2025 Apr 1;14(7):1073. doi: 10.3390/plants14071073.
Root-knot nematodes (RKNs, spp.) have become the major yield-limiting biological factor in common bean production in many warmer-climate regions such as the south of Europe. Broadening the genetic base of resistance in elite common bean cultivars is the most effective and environmentally friendly method for managing this disease. Toward this goal, F, F, and F populations from crosses between susceptible snap beans (Helda and Perona) and the resistant Ouro Negro cultivar were phenotyped for and -induced root-galling (GI) and egg mass production (EM) in controlled growth chamber infection assays. F progenies showed a susceptible response to both RKN isolates, with high GI and EM values, indicating a recessive inheritance of nematode resistance. The estimates for broad-sense heritability for GI and EM in the F Helda × Ouro Negro population infected with were 0.62 and 0.54, respectively. RKN resistance in Ouro Negro is largely controlled by partial to overdominant genetic effects and that susceptibility factor leads recessive resistance. The minimum number of genes involved in nematode resistance was estimated to be about two or three. In agreement, genetic analysis of F segregating populations supported duplicate recessive epistasis as the inheritance pattern involved in the resistance provided by the Ouro Negro cultivar. Ouro Negro is an important resource for broadening RKN resistance in elite common bean cultivars.
根结线虫已成为欧洲南部等许多气候较温暖地区菜豆生产中主要的产量限制生物因素。拓宽优良菜豆品种的抗性遗传基础是防治这种病害最有效且环保的方法。为实现这一目标，在可控生长室感染试验中，对感病的食荚菜豆（Helda和Perona）与抗病品种Ouro Negro杂交产生的F1、F2和F3群体进行了表型分析，测定了南方根结线虫和爪哇根结线虫诱导的根瘿（GI）和卵块产量（EM）。F1后代对两种根结线虫分离株均表现出感病反应，GI和EM值较高，表明线虫抗性为隐性遗传。在感染南方根结线虫的F2 Helda×Ouro Negro群体中，GI和EM的广义遗传力估计值分别为0.62和0.54。Ouro Negro的根结线虫抗性在很大程度上受部分显性到超显性遗传效应控制，感病因素导致隐性抗性。估计参与线虫抗性的基因最少约为两到三个。与此一致，F2分离群体的遗传分析支持重复隐性上位性是Ouro Negro品种提供的抗性所涉及的遗传模式。Ouro Negro是拓宽优良菜豆品种根结线虫抗性的重要资源。
