Mello Victor Carlos, de Brito Giovanna Oliveira, Radicchi Marina Arantes, Florêncio Isadora, Piau Tathyana Benetis, Ferreira Eduardo Antonio, de Azevedo Chang Leonardo Fróes, Silveira Ariane Pandolfo, Simões Marina Mesquita, de Paiva Karen Letycia Rodrigues, Salviano Santos Mac-Kedson Medeiros, Alves Nicole Santana, Grisolia Cesar Koppe, Báo Sônia Nair, Gris Eliana Fortes
Cooil Cosmetics, Brasília 72622-401, Brazil.
Laboratory of Microscopy and Microanalysis, Department of Cell Biology, Institute of Biological Sciences, University of Brasília, Brasília 70910-900, Brazil.
Antioxidants (Basel). 2025 Feb 28;14(3):290. doi: 10.3390/antiox14030290.
This study explores the development and characterization of lipid nanostructures (NLCs) containing natural deep eutectic solvents (NaDESs) derived from taperebá peel extract (), a by-product rich in bioactive phenolic compounds, including ellagic acid and quercetin. The taperebá extract exhibited a high polyphenol content (2623 mg GAE/L) and notable antioxidant activity, as demonstrated by DPPH (258 mM TEAC/100 mL) and ABTS (495 mM TEAC/100 mL) assays. NLCs were developed using NaDESs to enhance the stability and bioavailability of the antioxidant compounds. Physicochemical characterization confirmed the formation of stable, nanometric, and monodispersed formulations with efficient encapsulation. Biological evaluation of the NLC-TAP-NaDES formulation demonstrated its remarkable capacity to mitigate oxidative stress in cells subjected to HO-induced ROS generation. Fluorescence imaging revealed a significant reduction in intracellular ROS levels in treated cells compared to untreated controls, confirming the antioxidant efficacy of the formulation. This outcome underscores the synergy between NaDESs and NLC systems in protecting and delivering phenolic compounds. This study highlights the potential of utilizing underexplored by-products, such as taperebá peels, to develop sustainable and effective antioxidant delivery systems. The NLC-TAP-NaDES platform combines nanotechnology with green chemistry principles, presenting significant implications for the treatment of oxidative stress-related conditions and broader applications in pharmaceutical and nutraceutical sciences. These findings contribute to advancing sustainable innovations in antioxidant therapies, leveraging the dual benefits of bioeconomy and high-performance nanomaterials.
本研究探索了含有源自塔佩拉巴果皮提取物的天然低共熔溶剂（NaDESs）的脂质纳米结构（NLCs）的开发与表征，塔佩拉巴果皮提取物是一种富含生物活性酚类化合物（包括鞣花酸和槲皮素）的副产品。塔佩拉巴提取物表现出高多酚含量（2623 mg GAE/L）和显著的抗氧化活性，如通过DPPH（258 mM TEAC/100 mL）和ABTS（495 mM TEAC/100 mL）测定所证明的。使用NaDESs开发NLCs以提高抗氧化化合物的稳定性和生物利用度。物理化学表征证实形成了具有有效包封的稳定、纳米级和单分散的制剂。对NLC-TAP-NaDES制剂的生物学评估表明，其在减轻受HO诱导的ROS产生的细胞中的氧化应激方面具有显著能力。荧光成像显示，与未处理的对照相比，处理细胞中的细胞内ROS水平显著降低，证实了该制剂的抗氧化功效。这一结果强调了NaDESs与NLC系统在保护和递送酚类化合物方面的协同作用。本研究突出了利用未充分探索的副产品（如塔佩拉巴果皮）开发可持续且有效的抗氧化剂递送系统的潜力。NLC-TAP-NaDES平台将纳米技术与绿色化学原理相结合，对氧化应激相关病症的治疗以及在制药和营养科学中的更广泛应用具有重要意义。这些发现有助于推动抗氧化疗法的可持续创新，利用生物经济和高性能纳米材料的双重优势。
