Gad-El Karim M M, Ramanujam V M, Legator M S
Xenobiotica. 1985 Mar;15(3):211-20. doi: 10.3109/00498258509045351.
A sensitive h.p.l.c. method is described which separated urinary metabolites from benzene-treated male CD-1 mice. Phenol, trans,trans-muconic acid and quinol in the 48 h urine accounted, respectively, for 12.8-22.8, 1.8-4.7 and 1.5-3.7% of the orally administered single dose of benzene (880, 440 and 220 mg/kg body wt.). Catechol occurred in trace amounts. Ascorbic acid was used to adjust urine pH and increase the extraction efficiency of metabolites, especially muconic acid. It allowed an accurate estimation of quinol by preventing its auto-oxidation. trans,trans-Muconic acid was identified and was unique to benzene as none was detected in urine of mice dosed orally with phenol, catechol or quinol (250, 150 and 200 mg/kg, respectively). The potential existence of a toxic benzene metabolite in the form of an aldehyde precursor of muconic acid in vivo is discussed.
本文描述了一种灵敏的高效液相色谱法，用于分离经苯处理的雄性CD-1小鼠尿液中的代谢产物。48小时尿液中的苯酚、反,反-粘康酸和对苯二酚分别占口服单剂量苯（880、440和220mg/kg体重）的12.8 - 22.8%、1.8 - 4.7%和1.5 - 3.7%。儿茶酚含量微量。使用抗坏血酸调节尿液pH值并提高代谢产物的提取效率，尤其是粘康酸。通过防止对苯二酚自动氧化，可对其进行准确测定。已鉴定出反,反-粘康酸，它是苯所特有的，因为分别口服苯酚、儿茶酚或对苯二酚（250、150和200mg/kg）的小鼠尿液中未检测到该物质。本文还讨论了体内以粘康酸醛前体形式存在有毒苯代谢产物的可能性。
