Beynen A C, Katan M B
Am J Clin Nutr. 1985 Aug;42(2):317-22. doi: 10.1093/ajcn/42.2.317.
The fatty acid composition of fat tissue is a valid indicator of the fatty acid composition of the diet over the preceding 1 to 3 yr. Here we describe a rapid method for sampling of buttock fat without anesthesia. On average, 35 mg can be obtained using a common blood-sampling system. Sampling of 40 subjects takes about 3 h. The procedure caused no more anxiety or discomfort than a routine venipuncture. Application in about 500 subjects has as yet yielded no infectious or other complications. With regard to the fatty acid composition of the biopsy, sampling site (left versus right buttock) was not found to be a source of error. The biopsies could be stored for periods up to 1.5 yr without changes in the fatty acid profile. With this method one can obtain biopsies from a large number of subjects and determine objectively the long-term fatty acid composition of their diet.
脂肪组织的脂肪酸组成是过去1至3年饮食中脂肪酸组成的有效指标。在此，我们描述一种无需麻醉即可采集臀部脂肪的快速方法。平均而言，使用普通采血系统可获取35毫克脂肪。对40名受试者进行采样大约需要3小时。该操作引起的焦虑或不适并不比常规静脉穿刺更多。应用于约500名受试者至今未产生感染或其他并发症。关于活检的脂肪酸组成，未发现采样部位（左臀部与右臀部）是误差来源。活检样本可保存长达1.5年而脂肪酸谱无变化。通过这种方法，可以从大量受试者中获取活检样本，并客观地确定他们饮食中的长期脂肪酸组成。
