Pană Ioan-Ovidiu, Ciorîță Alexandra, Boca Sanda, Guțoiu Simona, Kacso Irina, Miclăuș Maria Olimpia, Grad Oana, Gherman Ana Maria Raluca, Leostean Cristian, Suciu Maria
National Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donat Street, 400293 Cluj-Napoca, Romania.
Electron Microscopy Center C. Craciun, Faculty of Biology and Geology, Babeș-Bolyai University, 5-7 Clinicilor Street, 400006 Cluj-Napoca, Romania.
Biomimetics (Basel). 2025 Mar 26;10(4):203. doi: 10.3390/biomimetics10040203.
Understanding cellular interaction with nanomaterials represents a subject of great interest for the validation of new diagnostic and therapeutic tools. A full characterization of a designed product includes the evaluation of its impact on specific biological systems, including the study of cell behavior as a response to that particular interaction. Copper and copper-based nanoparticles (CuO NPs) have emerged as valuable building blocks for various biomedical applications such as antibacterial and disinfecting agents for infectious diseases, and the evaluation of the metabolism of food, including the iron required for proteins and enzymes or as drug delivery systems in cancer therapy. In this study, the biological impact of manganese-doped crystalline copper oxide (CuO:Mn) nano-platelets on human normal BJ fibroblasts and human A375 skin melanoma was assessed. The particles were synthesized at room temperature via the hydrothermal method. A complete physicochemical characterization of the materials was performed by employing various techniques including X-ray diffraction, electron microscopy, X-Ray photoelectron spectroscopy, and dynamic light scattering. Morphological investigations revealed a flat structure with nearly straight edges, with sizes spanning in the nanometer range. XRD analysis confirmed the formation of the CuO phase with good crystallinity, while XPS provided insights into the Mn doping. The findings indicate that nano-platelets interact with cells actively by mediating essential molecular processes. The exogenous manganese triggers increased MnSOD production in mitochondria, compensating ROS produced by external stress factors (Cu ions), and mimics the endogenous SODs production, which compensates internal ROS production as it normally results from cell biochemistry. The effect is differentiated in normal cells compared to malignant cells and deserves investigation.
了解细胞与纳米材料的相互作用是验证新型诊断和治疗工具的一个极具吸引力的课题。对设计产品的全面表征包括评估其对特定生物系统的影响，包括研究细胞对这种特定相互作用的反应行为。铜及铜基纳米颗粒（CuO NPs）已成为各种生物医学应用中有价值的构建模块，如用于传染病的抗菌和消毒剂、食物代谢评估（包括蛋白质和酶所需的铁）或癌症治疗中的药物递送系统。在本研究中，评估了锰掺杂的结晶氧化铜（CuO:Mn）纳米片对人正常BJ成纤维细胞和人A375皮肤黑色素瘤的生物学影响。通过水热法在室温下合成了这些颗粒。采用包括X射线衍射、电子显微镜、X射线光电子能谱和动态光散射等各种技术对材料进行了完整的物理化学表征。形态学研究揭示了一种边缘近乎笔直的扁平结构，尺寸在纳米范围内。XRD分析证实了具有良好结晶度的CuO相的形成，而XPS提供了有关锰掺杂的见解。研究结果表明，纳米片通过介导基本分子过程与细胞积极相互作用。外源性锰会促使线粒体中MnSOD的产生增加，补偿外部应激因素（铜离子）产生的ROS，并模拟内源性SOD的产生，内源性SOD可补偿细胞生物化学正常产生的内部ROS。与恶性细胞相比，这种效应在正常细胞中有所不同，值得研究。
