Wissel Emily F, Chien Hsin-Yuan, Wei Ke-Hsuan, Lee Yi-Chan, Ullah Kiramat, Hsieh Patrick C H
Institute of Biomedical Sciences, Academia Sinica, Taipei, 115, Taiwan.
International Graduate Program in Molecular Medicine, National Yang Ming Chiao Tung University, Hsinchu, Taiwan.
Sci Rep. 2025 Apr 25;15(1):14576. doi: 10.1038/s41598-025-96695-z.
Heart Failure (HF) impacts approximately 64 million people globally. While overall incidence of HF is relatively stable across countries, the overall number of HF patients is increasing due to aging populations. Many articles examine the microbiome in HF, however, studies from humans have not been analyzed systematically. The aim of this meta-analysis is to bridge this gap by analyzing previously published data on human HF patients with untargeted metabolomics to understand whether microbially-mediated metabolites are consistently important for HF status. A systematic survey of the literature identified 708 articles discussing HF, the microbiome, and metabolomics. Of these, 82 were primary studies of HF patients, 61 studied human adults, 23 included an untargeted metabolomics measure, and 3 studies had data that was usable and publicly accessible. These studies include a GCMS study from stool, NMR of saliva and exhaled breath condensate, and LCMS from left ventricle of HF patients undergoing transplantation and unused donor hearts. Significant differences were observed from PCA between HF and controls for stool and left ventricle, but not saliva or EBC samples. OPLS-DA was conducted for stool and ventricle samples, and further revealed significant group differences. Univariate testing with FDR correction revealed 8 significant microbially-relevant metabolites (p < 0.005 after correction), most notably asparagine from left ventricle and 2-methylbutyryl carnitine from stool. Though there is much discussion of the microbiome in health outcomes in HF, there is limited research from human populations. Some microbial co-metabolites from both stool and heart were significantly associated with HF.
心力衰竭（HF）在全球约影响6400万人。虽然各国HF的总体发病率相对稳定，但由于人口老龄化，HF患者的总数正在增加。许多文章研究了HF中的微生物组，然而，来自人类的研究尚未得到系统分析。本荟萃分析的目的是通过分析先前发表的关于HF患者的非靶向代谢组学数据来填补这一空白，以了解微生物介导的代谢物是否始终对HF状态具有重要意义。一项系统的文献调查确定了708篇讨论HF、微生物组和代谢组学的文章。其中，82篇是对HF患者的初步研究，61篇研究了成年人，23篇纳入了非靶向代谢组学测量，3项研究的数据可用且可公开获取。这些研究包括一项来自粪便的气相色谱 - 质谱联用（GCMS）研究、唾液和呼出气冷凝物的核磁共振（NMR）研究，以及来自接受移植的HF患者左心室和未使用的供体心脏的液相色谱 - 质谱联用（LCMS）研究。在粪便和左心室样本中，HF组与对照组之间通过主成分分析（PCA）观察到显著差异，但唾液或呼出气冷凝物样本中未观察到。对粪便和心室样本进行了正交偏最小二乘法判别分析（OPLS-DA），进一步揭示了显著的组间差异。经错误发现率（FDR）校正的单变量检验揭示了8种与微生物相关的显著代谢物（校正后p < 0.005），最显著的是来自左心室的天冬酰胺和来自粪便的2-甲基丁酰肉碱。尽管在HF的健康结局中对微生物组有很多讨论，但来自人群的研究有限。来自粪便和心脏的一些微生物共代谢物与HF显著相关。
