Panova Natalina, Gerasimova Anelia, Tumbarski Yulian, Ivanov Ivan, Todorova Mina, Dincheva Ivayla, Gentscheva Galia, Gledacheva Vera, Slavchev Valeri, Stefanova Iliyana, Petkova Nadezhda, Nikolova Stoyanka, Nikolova Krastena
Department of Physics and Biophysics, Faculty of Pharmacy, Medical University of Varna, 84 Tzar Osvbod-Tel, 9000 Varna, Bulgaria.
Department of Chemistry, Faculty of Pharmacy, Medical University of Varna, 84 Tzar Osvoboditel, 9000 Varna, Bulgaria.
Life (Basel). 2025 Apr 1;15(4):583. doi: 10.3390/life15040583.
is one of the most famous plants used for medicinal purposes. Its leaf extracts have antimicrobial activity and antioxidant activities, and reduce swelling in ulcers.
The present article aimed to determine the metabolic profile of Moringa oleifera leaf extracts from two samples originating from India and to evaluate in vitro and ex vivo their biological activities.
The antioxidant properties of leaf extracts (methanol, 50% ethanol, and 70% ethanol) were studied in vitro for antimicrobial, anti-inflammatory, and antioxidant activities. The ex vivo contractile effects of the extracts were determined by assaying circularly isolated smooth muscle (SM) strips from a rat's stomach.
The obtained results indicated that one of the samples had amino acid and organic acid content approximately twice that of the second sample. In all the tests, the 50% ethanol extract of both samples showed better antioxidant activity (209 mM TE 100 g for the DPPH method). The 70% ethanolic extract of Sample 1 exhibited the strongest antimicrobial activity, inhibiting Gram-positive , , and . The 50% ethanolic extract of both samples exhibited the highest anti-inflammatory activity, demonstrating a twice better effect than the reference drug diclofenac. Finally, the pre-incubation of SM preparations with both samples significantly enhanced the ACh-induced contractile response, increasing it by 134% and 111%, respectively.
The findings suggested potential applications of as a suitable candidate for antimicrobial, antioxidant, anti-inflammatory, and antispasmodic supplementation for alternative pharmaceutical and nutraceutical products.
是最著名的药用植物之一。其叶提取物具有抗菌活性和抗氧化活性，并能减轻溃疡肿胀。
本文旨在确定来自印度的两个样品的辣木叶提取物的代谢谱，并在体外和离体条件下评估其生物活性。
研究了叶提取物（甲醇、50%乙醇和70%乙醇）的抗氧化性能，以检测其抗菌、抗炎和抗氧化活性。通过测定大鼠胃中环状分离的平滑肌（SM）条带来确定提取物的离体收缩作用。
所得结果表明，其中一个样品的氨基酸和有机酸含量约为另一个样品的两倍。在所有测试中，两个样品的50%乙醇提取物均表现出较好的抗氧化活性（DPPH法为209 mM TE 100 g）。样品1的70%乙醇提取物表现出最强的抗菌活性，可抑制革兰氏阳性菌、和。两个样品的50%乙醇提取物均表现出最高的抗炎活性，其效果比参比药物双氯芬酸好两倍。最后，将两个样品与SM制剂预孵育可显著增强乙酰胆碱诱导的收缩反应，分别使其增加134%和111%。
研究结果表明，作为抗菌、抗氧化、抗炎和解痉补充剂的合适候选物，在替代药物和营养保健品方面具有潜在应用价值。
