Zhou Yulong, Yao Yonghua, Zhang Furong, Yu Ning, Wang Binqiang, Tian Bing
Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, China.
Institute of Biophysics, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
Microorganisms. 2025 Mar 26;13(4):747. doi: 10.3390/microorganisms13040747.
Constructions of self-assembled protein nanocages for enzyme immobilization and cargo transport are very promising in biotechnology fields such as natural product biosynthesis. Here, we present an engineered isopentenyl pyrophosphate (IPP) synthetic nanocage with multiple enzymes for lycopene production in bacteria. The enzymes involved in IPP biosynthesis (ScCK, AtIPK, and MxanIDI) were assembled onto the exterior of an engineered protein cage based on α-carboxysome. The IPP synthetic nanocage was co-expressed with CrtE/CrtB/CrtI in . This approach increased the metabolic flux and resulted in a 1.7-fold increase in lycopene production in the engineered . compared with the control strain. The results provide insights into the immobilization and assembling of IPP biosynthetic enzymes in protein nanocages, which serve as a powerful tool for achieving efficient synthesis of lycopene.
用于酶固定化和货物运输的自组装蛋白质纳米笼的构建在天然产物生物合成等生物技术领域非常有前景。在此，我们展示了一种工程化的异戊烯基焦磷酸（IPP）合成纳米笼，其带有多种酶，用于在细菌中生产番茄红素。参与IPP生物合成的酶（ScCK、AtIPK和MxanIDI）被组装到基于α-羧酶体的工程化蛋白质笼的外部。IPP合成纳米笼与CrtE/CrtB/CrtI在[具体菌株]中共表达。这种方法增加了代谢通量，导致工程化[具体菌株]中的番茄红素产量比对照菌株增加了1.7倍。这些结果为IPP生物合成酶在蛋白质纳米笼中的固定化和组装提供了见解，蛋白质纳米笼是实现番茄红素高效合成的有力工具。
