Tripathi Garima, Park Myeongki, Park Seong-Su, Kim Hai-Doo, Lee Byoung-Ryol, Lee Byong-Taek
Institute of Tissue Regeneration, Soonchunhyang University, Cheonan 31204, South Korea.
Department of Regenerative Medicine, College of Medicine, Soonchunhyang University, Cheonan 31204, South Korea.
ACS Omega. 2025 Apr 11;10(15):14961-14971. doi: 10.1021/acsomega.4c10336. eCollection 2025 Apr 22.
The current study aimed to create a modified injectable bone substitute (IBS) with improved osteogenic characteristics. To enhance the cement injectability and release properties of curcumin in vitro, we incorporated curcumin into β-tricalcium phosphate (β-TCP) to create the composite supplemented with sintered porous β-TCP microspheres, which maintained good injectability with an initial setting time of 5.0 ± 1.3 min and a mechanical strength of 20.67 ± 1.3 MPa for bone cements. The differentiation potential of preosteoblast (MC3T3-E1) to osteoblasts was significantly boosted by incorporating curcumin, which triggered Col I, upregulated OPN expression, and boosted alkaline phosphatase activity. Bone regeneration was hastened after the in vivo implantation of curcumin-modified brushite (BR4P2C) cement into the implanted femoral defects, and the rate of cement disintegration was accelerated. As a result, the translational application of modified BR4P2C in preliminary research has the potential to serve as an IBS with curcumin, which has an exceptional ability to repair bone lesions.
本研究旨在制备一种具有改善成骨特性的改良型可注射骨替代物（IBS）。为了在体外提高姜黄素在骨水泥中的可注射性和释放性能，我们将姜黄素掺入β-磷酸三钙（β-TCP）中，制成添加了烧结多孔β-TCP微球的复合材料，该骨水泥初始凝固时间为5.0±1.3分钟，机械强度为20.67±1.3MPa，保持了良好的可注射性。掺入姜黄素可显著提高前成骨细胞（MC3T3-E1）向成骨细胞的分化潜能，姜黄素可触发I型胶原（Col I），上调骨桥蛋白（OPN）表达，并提高碱性磷酸酶活性。将姜黄素改性的透钙磷石（BR4P2C）骨水泥植入股骨缺损后，可加速骨再生，加快骨水泥的降解速度。因此，改性BR4P2C在初步研究中的转化应用有可能作为一种含姜黄素的IBS，具有修复骨损伤的卓越能力。
