An Hao, Kim Yu-Kyoung, Jang Yong-Seok, Lee Min-Ho
Department of Dental Biomaterials, Institute of Biodegradable Materials, School of Dentistry, Jeonbuk National University, Jeon-Ju 54896, South Korea.
ACS Omega. 2025 Apr 10;10(15):14593-14601. doi: 10.1021/acsomega.4c06731. eCollection 2025 Apr 22.
Due to the low bioactivity of titanium implants, the extended bone integration process after implantation substantially heightens the risk of inflammation, a primary cause of implant failure. To mitigate inflammatory responses and enhance bone integration between the implant and bone tissue, based on prior research that applied calcium phosphate (CaP) on titanium surfaces, we employed electrospraying technology to develop a drug-loaded polycaprolactone/silk fibroin/polydopamine (PCL/SF/PDA) composite coating as the second layer on top of the calcium phosphate deposition. The surface morphologies of the CaP deposits and composite coatings were characterized by SEM. The SF/PDA gel significantly increased the adhesion of the coating, thereby enhancing its clinical application potential. All materials exhibited excellent biodegradability, and their superior biocompatibility was confirmed through cell assays. Following in vitro experiments, in vivo studies were conducted using a rat cranial defect model. Micro-CT results and staining demonstrated that CaP deposition significantly accelerated bone integration between the titanium substrate and bone, while the drug-loaded polymer coating notably improved the inflammatory environment at the defect site. These findings offer new insights into the development of titanium implants.
由于钛植入物的生物活性较低，植入后延长的骨整合过程会大幅增加炎症风险，而炎症是植入失败的主要原因。为了减轻炎症反应并增强植入物与骨组织之间的骨整合，基于先前在钛表面应用磷酸钙（CaP）的研究，我们采用电喷雾技术开发了一种载药聚己内酯/丝素蛋白/聚多巴胺（PCL/SF/PDA）复合涂层，作为磷酸钙沉积层之上的第二层。通过扫描电子显微镜（SEM）对CaP沉积物和复合涂层的表面形态进行了表征。SF/PDA凝胶显著提高了涂层的附着力，从而增强了其临床应用潜力。所有材料均表现出优异的生物降解性，并且通过细胞实验证实了它们具有卓越的生物相容性。在体外实验之后，使用大鼠颅骨缺损模型进行了体内研究。微型计算机断层扫描（Micro-CT）结果和染色表明，CaP沉积显著加速了钛基底与骨之间的骨整合，而载药聚合物涂层显著改善了缺损部位的炎症环境。这些发现为钛植入物的开发提供了新的见解。
