OBJECTIVE: Zoonotic cutaneous leishmaniasis is a common including endemic infectious disease in many parts of the world and Iran. Due to wide therapeutic applications, the anti-leishmanial effect of the hydroalcoholic extract of its leaves (L), roots (R) and seeds (S) has been investigated in this research. MATERIALS AND METHODS: The leaves, seeds and roots of the greater burdock plant were extracted. In the phase, its cytotoxic and anti-leishmanial effects on promastigote and amastigote forms of were investigated. In the in vivo stage, the leishmaniasis mouse model was dosed with concentrations of 50, 100, and 200 mg/kg and the liver and spleen parasite burden was checked. RESULTS: The results of this research in the phase showed that the antileishmanial effect of the hydroalcoholic extract of leaves, roots and seeds on the promastigote and amastigote forms of has a significant relationship with the increase in the concentration of the extract (for all p≤0.001). Also, exposure time and interaction effect of concentration and exposure time were significant. In the phase the significant effect of the increase in concentration (L: p≤0.001, R: p=0.02, S: p=0.03), exposure time (L: p≤0.001, R: p≤0.001, S: p≤0.01) and the interaction effect of these two factors (L: p=0.002, R: p≤0.001, S: p≤0.001) on reducing the size of the wound was shown. CONCLUSION: The investigation established that hydroalcoholic extract of the leaves, roots, and seeds of the greater burdock in high concentration exhibited beneficial inhibitory effects on the leishmanial lesions.