Lima Erica Tirzah S, Santos Victoria L S, Mota Wanessa J S, Martins Frederico S, de Albuquerque-Junior Ricardo L C, Santos André L S, Oliveira Simone S C, de Lima Jéssica A, Santos Adriana de Jesus, Dos Santos Cochiran P, Jain Sona, Souto Eliana B, Cardoso Juliana C, Severino Patrícia
Institute of Research and Technology, University Tiradentes, Aracaju, Brazil.
School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, São Paulo, Brazil.
Chem Biodivers. 2025 Sep;22(9):e02348. doi: 10.1002/cbdv.202402348. Epub 2025 May 20.
This study describes the green synthesis, characterization, and biological evaluation of silver nanoparticles (AgNPs) obtained from green propolis (AgNPs-PRO). Before nanoparticles synthesis, a hydroethanolic green propolis extract (GPE) was obtained through ultrasound-assisted extraction and characterized by high-performance liquid chromatography, revealing the artepilin C as the most abundant phenolic compound in its composition, followed by 4,5-dicaffeoylquinic acid and drupanin. The analysis of synthesized AgNPs by UV-Vis spectroscopy showed a characteristic absorption band at 430 nm. Dynamic light scattering analysis revealed mean hydrodynamic particle sizes ranging from 88 to 115 nm, with a polydispersity index between 0.229 ± 0.006 and 0.365 ± 0.054. Fourier-transform infrared spectroscopy confirmed that functional groups present in GPE contribute to the reduction and stabilization of AgNPs. Differential scanning calorimetry and transmission electron microscopy confirmed that AgNPs were obtained. GPE showed leishmanicidal activity against promastigote forms of Leishmania amazonensis, with a half-maximal inhibitory concentration (IC) of 11.87 µg/mL and a selectivity index (SI) of 12.52. Antibacterial activity of the AgNPs, assessed via the disk diffusion method, revealed inhibition zones against Escherichia coli (Gram-negative), Staphylococcus aureus (Gram-positive), and Candida albicans strains. The HET-CAM test indicated no signs of irritation, suggesting the biocompatibility of the developed AgNPs.
本研究描述了从绿色蜂胶中获得的银纳米颗粒（AgNPs-PRO）的绿色合成、表征及生物学评价。在纳米颗粒合成之前，通过超声辅助提取获得了水乙醇绿色蜂胶提取物（GPE），并通过高效液相色谱进行了表征，结果显示artepilin C是其组成中含量最丰富的酚类化合物，其次是4,5-二咖啡酰奎宁酸和德鲁帕宁。通过紫外-可见光谱对合成的AgNPs进行分析，结果显示在430 nm处有一个特征吸收带。动态光散射分析表明，平均流体动力学粒径范围为88至115 nm，多分散指数在0.229±0.006和0.365±0.054之间。傅里叶变换红外光谱证实，GPE中存在的官能团有助于AgNPs的还原和稳定。差示扫描量热法和透射电子显微镜证实获得了AgNPs。GPE对亚马逊利什曼原虫的前鞭毛体形式显示出杀利什曼活性，半数最大抑制浓度（IC）为11.87 μg/mL，选择性指数（SI）为12.52。通过纸片扩散法评估的AgNPs的抗菌活性显示出对大肠杆菌（革兰氏阴性）、金黄色葡萄球菌（革兰氏阳性）和白色念珠菌菌株的抑菌圈。HET-CAM试验表明没有刺激迹象，这表明所开发的AgNPs具有生物相容性。
