Chirilă Mihaela, Suciu Ioana, Ionescu Ecaterina, Popescu Ionela Daniela, Codorean Eleonora, Codrici Elena, Chirilă Lucian, Amza Oana, Dimitriu Bogdan, Nichita Cornelia
Department of Endodontics, Faculty of Dental Medicine, University of Medicine and Pharmacy "Carol Davila", Bucharest, Romania.
Department of Orthodontics and Dento-Facial Orthopedics, Faculty of Dental Medicine, University of Medicine and Pharmacy "Carol Davila", Bucharest, Romania.
Int J Dent. 2025 Apr 22;2025:6661398. doi: 10.1155/ijod/6661398. eCollection 2025.
This study aims to test the cytoprotective effect of L. fruit extracts on cell viability and its suitability as a transport medium for avulsed teeth. . Two L. fruit extracts were synthesized, E1 and E2. The E1 extract was composed of fresh mulberry fruits, whereas the E2 extract was made from dehydrated fruits. The cytoprotective effect of the E1 and E2 extracts was determined using MTS testing over 5000 and 10,000 cells, after a 48 h incubation period, and sampling times of 1 h and 3 h. The concentrations tested were 50 μg/mL (E1A and E2A), 150 μg/mL (E1B and E2B), and 250 μg/mL (E1C and E2C). The data was analyzed using the one-way analysis of variance (ANOVA) test ( < 0.05) and post hoc LSD (least significant difference) analysis ( < 0.1). The post hoc LSD analysis based on the concentration of the E1 extract showed statistically differences ( < 0.1), and for the E2 extract, the results were highly significant ( = 0.011). As a function of concentration between the populations, there are significant differences between E1A and E1B ( = 0.071) and between E1B and E1C ( = 0.084), and statistically insignificant differences between E1A and E1C ( = 0.919). Significant differences were also detected between the E2A and E2B extracts ( = 0.047) and between E2B and E2C ( = 0.004). . Analyzing the preliminary results of our study, we can conclude that the L. extract can be considered a potential transport medium for avulsed teeth.
本研究旨在测试L.果实提取物对细胞活力的细胞保护作用及其作为脱位牙运输介质的适用性。合成了两种L.果实提取物，E1和E2。E1提取物由新鲜桑椹果实组成，而E2提取物由脱水果实制成。在48小时孵育期后，使用MTS测试在5000和10000个细胞上，以及1小时和3小时的采样时间，测定E1和E2提取物的细胞保护作用。测试的浓度为50μg/mL（E1A和E2A）、150μg/mL（E1B和E2B）和250μg/mL（E1C和E2C）。使用单因素方差分析（ANOVA）检验（<0.05）和事后LSD（最小显著差异）分析（<0.1）对数据进行分析。基于E1提取物浓度的事后LSD分析显示出统计学差异（<0.1），对于E2提取物，结果具有高度显著性（=0.011）。作为群体间浓度的函数，E1A和E1B之间（=0.071）以及E1B和E1C之间（=0.084）存在显著差异，而E1A和E1C之间（=0.919）的差异无统计学意义。在E2A和E2B提取物之间（=0.047）以及E2B和E2C之间（=0.004）也检测到显著差异。分析我们研究的初步结果，我们可以得出结论，L.提取物可被视为脱位牙的潜在运输介质。
