He Xin-Chen, Shao Jie-Xin, Zou Wei, Zhang Shu-Xiao, Zhu Li, Ji Meng-Cheng, Gu Cui-Hua, Yang Li-Yuan
School of Landscape Architecture, Zhejiang Agriculture and Forestry University, Hangzhou, 311300, Zhejiang, China.
Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, College of Biology and Agricultural Resources, Huanggang Normal University, Huanggang, 438000, China.
Genetica. 2025 May 3;153(1):17. doi: 10.1007/s10709-025-00233-8.
The protein phosphatase 2 C (PP2C) plays a crucial role in the growth and development of plants. However, limited information on the PP2C genes in Chimonanthus spp. is available. Therefore, the comprehensive genome-wide identification and analysis of the PP2Cs gene family is necessary in Chimonanthus spp. to provide basic information for further study. In this work, 93 members of the CsPP2C gene family and 85 members of the CpPP2C gene family were identified. Based on the phylogenetic analysis, 93 CsPP2Cs and 85 CpPP2Cs genes were classified into 13 subgroups. Based on RNA-Seq data, specific expression patterns of CsPP2Cs in different tissues were identified. CsPP2C28, CsPP2C55, and CsPP2C17 showed high expression during leaf senescence, and combined with cis-element analysis, it is speculated that they may participate in regulating plant senescence. CsPP2C47, CsPP2C27, CsPP2C42, and CsPP2C41 may play an important role in responding to abscisic acid during seed dormancy and germination. These results contribute to a deeper understanding of the functions of the CsPP2C gene family and CpPP2C gene family, and providing candidate genes for genetic engineering and breeding to enhance important traits such as stress resistance and growth development in C. salicifolius and C. praecox.
蛋白磷酸酶2C（PP2C）在植物的生长发育中起着至关重要的作用。然而，关于蜡梅属植物中PP2C基因的信息有限。因此，有必要对蜡梅属植物中的PP2C基因家族进行全基因组的综合鉴定和分析，为进一步研究提供基础信息。在这项工作中，鉴定出了93个蜡梅CsPP2C基因家族成员和85个夏蜡梅CpPP2C基因家族成员。基于系统发育分析，93个CsPP2C基因和85个CpPP2C基因被分为13个亚组。基于RNA测序数据，确定了CsPP2C基因在不同组织中的特异性表达模式。CsPP2C28、CsPP2C55和CsPP2C17在叶片衰老过程中表达较高，结合顺式元件分析，推测它们可能参与调控植物衰老。CsPP2C47、CsPP2C27、CsPP2C42和CsPP2C41可能在种子休眠和萌发过程中对脱落酸的响应中发挥重要作用。这些结果有助于更深入地了解CsPP2C基因家族和CpPP2C基因家族的功能，并为基因工程和育种提供候选基因，以增强柳叶蜡梅和蜡梅的抗逆性和生长发育等重要性状。
