Wu Shiwei, Kan Ying, Xiao Peng, Yang Yumeng, Li Honemei, Zhang Weifei
College of Materials and Chemistry, China Jiliang University, Hangzhou 310018, China.
Key Laboratory of Chemical Metrology and Applications on Nutrition, Health, State Administration for Market Regulation, Division of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing 100029, China.
Anal Methods. 2025 May 15;17(19):4027-4036. doi: 10.1039/d5ay00267b.
Donepezil is a drug used to treat Alzheimer's disease, and its efficacy is closely related to the concentration of the drug in the serum. Therefore, monitoring the concentration of donepezil in the serum is crucial for evaluating the treatment effect and avoiding adverse reactions. In this study, a new method for determining the concentration of donepezil in serum was established by combining magnetic molecularly imprinted polymers (MMIPs) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) technology. The prepared magnetic molecularly imprinted polymers were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and transmission electron microscopy (TEM) to confirm that the polymer had effective imprinting and good magnetic responsivity. The adsorption experiment showed that the MMIPs had a saturated adsorption capacity of 15.4 mg g for donepezil and the adsorption behavior followed the quasi-second-order kinetic model. The adsorption of donepezil by MMIPs was a chemical adsorption process. Finally, by combining LC-MS/MS technology, the method successfully achieved rapid, high selectivity, and high specificity separation and enrichment detection of donepezil in serum. The linear range of the method for donepezil in serum was 25-200 ng mL, the lowest detection limit was 0.33 ng mL, the recovery was 98.7-102.6%, and the relative standard deviation (RSD) was 1.2-4.5% ( = 3). The experiment confirmed that the method is simple and effective for detecting donepezil in serum.
多奈哌齐是一种用于治疗阿尔茨海默病的药物，其疗效与血清中药物浓度密切相关。因此，监测血清中多奈哌齐的浓度对于评估治疗效果和避免不良反应至关重要。在本研究中，通过结合磁性分子印迹聚合物（MMIPs）和液相色谱-串联质谱（LC-MS/MS）技术，建立了一种测定血清中多奈哌齐浓度的新方法。通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FT-IR）和透射电子显微镜（TEM）对制备的磁性分子印迹聚合物进行表征，以确认该聚合物具有有效的印迹和良好的磁响应性。吸附实验表明，MMIPs对多奈哌齐的饱和吸附容量为15.4 mg/g，吸附行为遵循准二级动力学模型。MMIPs对多奈哌齐的吸附是一个化学吸附过程。最后，结合LC-MS/MS技术，该方法成功实现了血清中多奈哌齐的快速、高选择性和高特异性分离与富集检测。该方法测定血清中多奈哌齐的线性范围为25-200 ng/mL，最低检测限为0.33 ng/mL，回收率为98.7-102.6%，相对标准偏差（RSD）为1.2-4.5%（n = 3）。实验证实该方法用于检测血清中的多奈哌齐简单有效。
