Zhou Juan, Wu Fang, Luo Yang, Chen Zhenfu, Long Donghua, Liu Hui, Luo Bo, Yan Rong, Wang Lingjun
Department of Parasitology, Zunyi Medical University, Zunyi 563000, China.
Department of Pathological Biology and Immunology, Zunyi Medical and Pharmaceutical College, Zunyi 563000, China.
Insects. 2025 Mar 26;16(4):346. doi: 10.3390/insects16040346.
After eight generations of laboratory selection with β-cypermethrin, a strain was cultivated that displayed a 10.04-fold increase in resistance (RS) relative to the susceptible strain (SS), with an estimation of heritability () of 0.34. Compared with the SS, the developmental duration of the eggs was significantly prolonged ( < 0.05); however, the pupal stage duration was shorter, with no statistically significant difference. Moreover, the levels of GSTs, CarEs, and CYP450 activity were notably higher in the RS than in the SS. In addition, the level of CarE and CYP450 activity in the RS was significantly higher in the midgut (MG), fat body (FB), and Malpighian tubules (MTs) compared to the SS; however, the GSTs showed no statistically significant difference in the MTs. These results suggest that 's resistance to β-cypermethrin could be selected rapidly and the decreases in the intrinsic rate of increase (r) observed in the RS are likely due to mutations in the detoxification enzyme genes under the strong selection pressure exerted by β-cypermethrin. The increased activity of GSTs, CarEs, and CYP450 was associated with β-cypermethrin resistance in the RS of The data reported herein provide a foundation for future studies on the mechanisms responsible for β-cypermethrin resistance in
在用高效氯氰菊酯进行八代实验室选育后，培育出了一个品系，相对于敏感品系（SS），该品系表现出10.04倍的抗性增加（RS），遗传力估计值为0.34。与SS相比，卵的发育历期显著延长（P<0.05）；然而，蛹期历期较短，无统计学显著差异。此外，RS中谷胱甘肽S-转移酶（GSTs）、羧酸酯酶（CarEs）的水平以及细胞色素P450活性显著高于SS。另外，与SS相比，RS中中肠（MG）、脂肪体（FB）和马氏管（MTs）中CarE的水平和CYP450活性显著更高；然而，GSTs在MTs中无统计学显著差异。这些结果表明，[具体物种]对高效氯氰菊酯的抗性可快速选育，并且在RS中观察到的内禀增长率（r）下降可能是由于在高效氯氰菊酯施加的强选择压力下解毒酶基因突变所致。GSTs、CarEs和CYP450活性的增加与[具体物种]RS中对高效氯氰菊酯的抗性相关。本文报道的数据为未来关于[具体物种]对高效氯氰菊酯抗性机制的研究奠定了基础。
