Buchanan R L, Hoover D G, Jones S B
Appl Environ Microbiol. 1983 Nov;46(5):1193-200. doi: 10.1128/aem.46.5.1193-1200.1983.
Evaluation of caffeine and a number of related methylxanthines indicated that the ability of the compound to inhibit growth and aflatoxin production by Aspergillus parasiticus is highly specific and does not involve an inhibition of cyclic AMP phosphodiesterase. Supplementation of the culture medium with purine bases, nucleosides, and nucleotides suggested that the inhibition of fungal growth could be partially overcome by adenine or guanine but that the purines had little effect on the inhibition of aflatoxin production. Likewise, increasing the levels of trace minerals did not overcome the inhibition of toxin production. Electron microscopic evaluation of caffeine-treated and -untreated cultures indicated that the compound produced observable changes in the ultrastructure of the fungus.
对咖啡因及多种相关甲基黄嘌呤的评估表明，该化合物抑制寄生曲霉生长及黄曲霉毒素产生的能力具有高度特异性，且不涉及对环磷酸腺苷磷酸二酯酶的抑制。在培养基中添加嘌呤碱、核苷和核苷酸表明，腺嘌呤或鸟嘌呤可部分克服对真菌生长的抑制，但这些嘌呤对黄曲霉毒素产生的抑制作用影响不大。同样，提高微量矿物质水平也无法克服对毒素产生的抑制。对经咖啡因处理和未处理的培养物进行电子显微镜评估表明，该化合物使真菌的超微结构产生了可观察到的变化。
