Rad Vahideh Farzam, Mohammadi Maryam, Moradi Ali-Reza
Department of Physics, Institute for Advanced Studies in Basic Sciences (IASBS), 45137-66731, Zanjan, Iran.
Biozentrum, University of Basel, Spitalstrasse 41, 4056, Basel, Switzerland.
Sci Rep. 2025 May 8;15(1):16068. doi: 10.1038/s41598-025-00955-x.
Particulate air pollution is associated with excess deaths and increases in hospital admissions because of cardiovascular and respiratory diseases. Several scientific studies and assessments have linked particulate pollution to a variety of health problems. In this paper, we provide a single cell in vitro analysis for the effect of the particles, which can enter into blood stream, on red blood cells (RBCs). The RBCs under experiment are incubated with [Formula: see text] particle as the most abundant air pollutants in big cities. The self-referencing digital holographic microscopy (DHM) in Gates' arrangement as a vibration-immune methodology is considered here for live visualization and quantitative analysis of the cells. DHM is a label-free and noninvasive method, therefore, suitable for quantitative and morphometric imaging of biological specimens in arbitrary time scales and at video rates. Single RBCs are immobilized by a blinking multiple optical trapping system integrated to the DHM system. Through post-process numerical reconstruction of the recorded digital holograms, the morphology changes of the pollution-exposed RBCs are tracked and expressed in terms of volume and several statistical morphometry parameters.
空气中的颗粒物污染与因心血管和呼吸系统疾病导致的超额死亡及住院人数增加有关。多项科学研究和评估已将颗粒物污染与多种健康问题联系起来。在本文中，我们对可进入血流的颗粒物对红细胞（RBC）的影响进行了单细胞体外分析。实验中的红细胞与大城市中最常见的空气污染物[化学式：见原文]颗粒一起孵育。这里采用盖茨配置的自参考数字全息显微镜（DHM）作为一种抗振动方法，用于对细胞进行实时可视化和定量分析。DHM是一种无标记且非侵入性的方法，因此适用于在任意时间尺度和视频速率下对生物标本进行定量和形态计量成像。单个红细胞通过集成到DHM系统的闪烁多光阱系统固定。通过对记录的数字全息图进行后处理数值重建，跟踪受污染红细胞的形态变化，并以体积和几个统计形态计量参数来表示。
