Heinegård D, Inerot S, Wieslander J, Lindblad G
Scand J Clin Lab Invest. 1985 Sep;45(5):421-7. doi: 10.1080/00365518509155238.
An enzyme linked immunosorbent assay for the quantification of proteoglycans or proteoglycan fragments in biological fluids is presented. The assay has been used to determine concentrations of articular cartilage proteoglycans and/or fragments thereof in synovial fluid in early stages of osteoarthrosis. Osteoarthrosis was induced in one knee joint of nine German wire haired pointers by transection of the anterior cruciate ligament. All dogs developed osteoarthrosis in terms of macroscopic as well as microscopic criteria. Attempts were made to aspirate synovial fluid, prior to surgery and at various times in the postoperative period, from the joint operated on as well as from the contralateral joint. Concentrations of proteoglycans (fragments) of one type of aggregating proteoglycan were significantly higher in the synovial fluid samples from the joint operated on compared to the contralateral joint (p less than 0.001), while the difference observed when the other type of aggregating proteoglycan was used as the test antigen was less significant (p less than 0.05). The concentration of proteoglycans (fragments) in synovial fluid samples from joints operated on was significantly higher after the operation when compared with the preoperative concentration (p less than 0.001). No correction has been made for the increased dilution due to hydrops of the joints operated on.
本文介绍了一种用于定量生物体液中蛋白聚糖或蛋白聚糖片段的酶联免疫吸附测定法。该测定法已用于在骨关节炎早期阶段测定滑液中关节软骨蛋白聚糖和/或其片段的浓度。通过切断前交叉韧带，在9只德国刚毛指示犬的一个膝关节中诱发骨关节炎。根据宏观和微观标准，所有犬均发生了骨关节炎。在手术前以及术后不同时间，尝试从手术关节以及对侧关节抽取滑液。与对侧关节相比，手术关节滑液样本中一种聚集蛋白聚糖的蛋白聚糖（片段）浓度显著更高（p<0.001），而当使用另一种聚集蛋白聚糖作为测试抗原时观察到的差异则不太显著（p<0.05）。与术前浓度相比，手术后手术关节滑液样本中蛋白聚糖（片段）的浓度显著更高（p<0.001）。未对手术关节积液导致的稀释增加进行校正。
