广藿香乙醇提取物对阿霉素诱导的3T3-L1成纤维细胞系衰老特征的影响
Patchouli Ethanol Extract Benth. Against Aging Profiles in Doxorubicin-Induced 3T3-L1 Fibroblast Cell Lines.
作者信息
Santoso Teguh, Khairani Astrid Feinisa, Bashari Muhammad Hasan, Ramadhanti Julia, Rohmawati Enny, Oktora Monika Pury, Wiraswati Hesti Lina
机构信息
Graduate School of Master Program in Anti-Aging and Aesthetic Medicine Faculty of Medicine, Universitas Padjadjaran Bandung West Java Indonesia.
Department of Biomedical Sciences Faculty of Medicine, Universitas Padjadjaran Bandung West Java Indonesia.
出版信息
Aging Med (Milton). 2025 Apr 18;8(2):156-163. doi: 10.1002/agm2.70014. eCollection 2025 Apr.
OBJECTIVES
This research aimed to investigate the potential of Benth's ethanolic extract (PCEE) as an antiaging agent.
METHODS
We treated 3T3-L1 cells induced by doxorubicin with PCEE and assessed them with antiaging markers such as gene and telomere length with polymerase chain reaction (PCR), or reactive oxygen species (ROS) accumulation with 2,7-dichlorofluorescein diacetate (DCFHDA).
RESULTS
This study demonstrates that PCEE can inhibit ROS accumulation, decrease the expression level of the senescence-associated β-galactosidase (SA-β-gal) and maintain telomere length in doxorubicin-induced 3T3-L1 cells.
CONCLUSION
These studies reveal that PCEE is a promising antiaging agent. An in vivo approach should be conducted for further research.
目的
本研究旨在探讨本氏乙醇提取物（PCEE）作为一种抗衰老剂的潜力。
方法
我们用PCEE处理阿霉素诱导的3T3-L1细胞，并用聚合酶链反应（PCR）检测基因和端粒长度等抗衰老标志物，或用二氯二氢荧光素二乙酸酯（DCFHDA）检测活性氧（ROS）积累来评估这些细胞。
结果
本研究表明，PCEE可以抑制阿霉素诱导的3T3-L1细胞中ROS的积累，降低衰老相关β-半乳糖苷酶（SA-β-gal）的表达水平，并维持端粒长度。
结论
这些研究表明PCEE是一种有前景的抗衰老剂。应采用体内实验方法进行进一步研究。
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