Tolba Heba E, Taha Yasser E, Fouad Nermeen A, Abdelaleem Omayma O
Department of Rheumatology and Rehabilitation, Faculty of Medicine, Fayoum University, Fayoum, Egypt.
Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Fayoum University, Fayoum, Egypt.
Clin Rheumatol. 2025 May 13. doi: 10.1007/s10067-025-07470-4.
Behçet's disease (BD) is characterized by a variety of clinical involvements and unpredictable courses of remission and exacerbation. BD cannot be diagnosed based on any particular laboratory, histopathologic, or genetic data. After excluding other possible causes, the diagnosis of BD is mostly made based on clinical manifestations. This research aims to assess the expression of miR-127 and miR-29 in Behçet patients and to correlate this expression with different disease manifestations and disease activity.
Seventy adult patients with Behçet disease and 30 matched controls were enlisted. Behçet's Disease Current Activity Form (BDCAF) was used to determine the patients' activity scores. Gene expressions of miR-127 and miR-29 were assessed using real-time PCR.
miR-29 was statistically significantly higher in cases when compared to controls with p < 0.001. Patients with oral ulcers, genital ulcers, and neurological manifestations had a significantly higher levels of miR-29b (p = 0.005, 0.008, 0.003, respectively). Expression level of miR-29b was higher in patients with active disease compared with those who were in inactive state (p = 0.003). Patients with high and moderate severity score had a statistically significant higher expression level of miR-29 when compared to patients with mild severity score. There was a statistically significant positive correlation between miR-29 and several study parameters including BDCAF (r = 0.440, p < 0.001) and severity score (r = 0.243, p = 0.043). miR-127 was statistically significantly lower in cases when compared to controls with p < 0.001. There were statistically significant negative correlations between miR-127 and each of ESR (r = - 0.361, p = 0.002) and BDCAF (r = - 0.350, p = 0.003).
miR-29 expression level was statistically significantly higher while miR-127 was statistically significantly lower in patients with Behçet disease compared with control individuals. Furthermore, miR-29 was significantly positively correlated whereas miR-127 was significantly negatively correlated with BDCAF denoting their association with disease activity. Key Points • Behçet's disease (BD) is a systemic inflammatory disease with uncertain etiology. There is currently no diagnostic test for Behçet disease. Several miRNAs have been identified as powerful diagnostic biomarkers in many diseases. • Measuring the expression levels of miR-29 and miR-127 in serum of sample of patients with Behçet disease was performed in the current study. • miR-29 expression level was statistically significantly higher while miR-127 was statistically significantly lower in patients with Behçet disease compared with control individuals. Furthermore, miR-29 was significantly positively correlated whereas miR-127 was significantly negatively correlated with BDCAF denoting their association with disease activity.
白塞病（BD）的特征是有多种临床症状，且缓解和加重过程不可预测。白塞病无法根据任何特定的实验室、组织病理学或基因数据进行诊断。在排除其他可能原因后，白塞病的诊断主要基于临床表现。本研究旨在评估白塞病患者中miR-127和miR-29的表达，并将这种表达与不同的疾病表现和疾病活动度相关联。
招募了70名成年白塞病患者和30名匹配的对照。使用白塞病当前活动形式（BDCAF）来确定患者的活动评分。使用实时PCR评估miR-127和miR-29的基因表达。
与对照组相比，miR-29在病例中的表达在统计学上显著更高，p < 0.001。患有口腔溃疡、生殖器溃疡和神经表现的患者miR-29b水平显著更高（分别为p = 0.005、0.008、0.003）。与处于非活动状态的患者相比，活动期疾病患者的miR-29b表达水平更高（p = 0.003）。与轻度严重程度评分的患者相比，高和中度严重程度评分的患者miR-29的表达水平在统计学上显著更高。miR-29与包括BDCAF（r = 0.440，p < 0.001）和严重程度评分（r = 0.243，p = 0.043）在内的几个研究参数之间存在统计学上显著的正相关。与对照组相比，miR-127在病例中的表达在统计学上显著更低，p < 0.001。miR-127与红细胞沉降率（ESR）（r = -0.361，p = 0.002）和BDCAF（r = -0.350，p = 0.003）中的每一个之间都存在统计学上显著的负相关。
与对照个体相比，白塞病患者中miR-29的表达水平在统计学上显著更高，而miR-127在统计学上显著更低。此外，miR-29与BDCAF呈显著正相关，而miR-127与BDCAF呈显著负相关，表明它们与疾病活动度相关。要点：• 白塞病（BD）是一种病因不明的全身性炎症性疾病。目前尚无白塞病的诊断测试。几种微小RNA（miRNA）已被确定为许多疾病中强大的诊断生物标志物。• 本研究对白塞病患者样本血清中miR-29和miR-127的表达水平进行了测量。• 与对照个体相比，白塞病患者中miR-29的表达水平在统计学上显著更高，而miR-127在统计学上显著更低。此外，miR-29与BDCAF呈显著正相关，而miR-127与BDCAF呈显著负相关，表明它们与疾病活动度相关。
