Wang Xinyue, Shao Yang, Zhou Xiaoling, Li Zheng, Liu Jingze, Tang Mingyao, Yang Yixin, Deng Liang
Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
Department of Pratacultural Science, College of Animal Science and Technology, Tarim University, Alaer 843300, China.
Animals (Basel). 2025 May 6;15(9):1337. doi: 10.3390/ani15091337.
The peripartum period is critical for breeding female donkeys (i.e., jennies) and ensuring the delivery of healthy neonatal foals. The gut microbiota deeply influences the host metabolism. This study aimed to investigate the dynamic changes in the gut microbiome during the peripartum period in jennies. Fresh fecal samples of eight adult jennies were collected at the following seven sampling time points: 21, 7, and 3 days prepartum (G21, G7, and G3) and 1, 3, 7, and 14 days postpartum (L1, L3, L7, and L14). Sequencing of the V4 hypervariable regions of the 16S rRNA genes was carried out using fecal samples to identify the differences in the microbiome across the peripartum period. Bacteroidota and Firmicutes were the most abundant bacterial phyla in the feces. and significantly increased in the L3 group compared to the G7 group ( < 0.05), and a decline trend was observed in L1 group around parturition. The genus , family Clostridiaceae, and order Clostridiales were considered to be biomarkers of the L3 group. Among the 25 functional pathways detected by Kyoto Encyclopedia of Genes and Genomes pathway analysis, beta lactam resistance, insulin resistance, and peptidases were the top three important pathways observed in the gut microbiota during the peripartum period in jennies. The gut microbial structure changed significantly at different time points during the peripartum period in jennies. These results contribute to a better understanding of the gut microbiota to ensure health care during important phases from late pregnancy to early lactation in jennies.
围产期对于繁殖母驴（即母驴）和确保健康新生驴驹的分娩至关重要。肠道微生物群深刻影响宿主代谢。本研究旨在调查母驴围产期肠道微生物组的动态变化。在以下七个采样时间点收集了八只成年母驴的新鲜粪便样本：产前21天、7天和3天（G21、G7和G3）以及产后1天、3天、7天和14天（L1、L3、L7和L14）。使用粪便样本对16S rRNA基因的V4高变区进行测序，以确定围产期微生物组的差异。拟杆菌门和厚壁菌门是粪便中最丰富的细菌门类。与G7组相比，L3组中 和 显著增加（<0.05），并且在分娩前后的L1组中观察到下降趋势。 属、梭菌科和梭菌目被认为是L3组的生物标志物。在京都基因与基因组百科全书通路分析检测到的25条功能通路中，β-内酰胺抗性、胰岛素抗性和肽酶是母驴围产期肠道微生物群中观察到的前三条重要通路。母驴围产期不同时间点的肠道微生物结构发生了显著变化。这些结果有助于更好地了解肠道微生物群，以确保母驴从妊娠晚期到哺乳早期的重要阶段的医疗保健。
