Abdelmoaty Mai M, Kadry Rana, Mosley R Lee, Gendelman Howard E
Department of Pharmacology and Experimental Neuroscience, College of Medicine, University of Nebraska Medical Center, NE 68198, USA.
Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, NE 68198, USA.
NeuroImmune Pharm Ther. 2024;3(2):129-137. doi: 10.1515/nipt-2024-0004. Epub 2024 Mar 6.
Alternative medicines commonly supplement or, at times, replace standard medical treatment. One area of increasing attention is disease-modifying medicines for neurodegenerative diseases. However, few such alternatives have been investigated thoroughly with an eye toward understanding mechanisms of action for clinical use. Medicinal mushrooms have important health benefits and pharmacological activities with anti-inflammatory, antioxidant, antibacterial, antiviral, immunomodulatory, digestive, cytoprotective, homeostatic, and neuroprotective activities. Edible mushrooms are known to play roles in preventing age-related diseases. Several studies have revealed that polysaccharides, terpenes, and phenolic compounds are chemical components derived from mushrooms with pharmacological activities. Due to limited effective protocols for mushroom protein extraction for proteomic studies, information about these medicinally related proteins and their biological functions remains enigmatic. Herein, we have performed proteomic studies of two mushroom species (agarikon) and (maitake). These studies serve to uncover a foundation for putative proteome-associated neuroprotective processes. The recovered proteins from both species show multiple cell-specific signaling pathways including unfolded protein response, and mitochondrial protein import as well as those linked to BAG2, ubiquitination, apoptosis, microautophagy, glycolysis, SNARE, and immunogenic cell signaling pathways. This study uncovered mushroom proteome-associated proteins which serve to better understand the structural and functional properties of mushrooms used as alternative medicines for broad potential health benefits.
替代医学通常补充或有时替代标准医学治疗。一个日益受到关注的领域是用于神经退行性疾病的疾病修饰药物。然而，很少有这样的替代药物经过深入研究以了解其临床使用的作用机制。药用蘑菇具有重要的健康益处和药理活性，具有抗炎、抗氧化、抗菌、抗病毒、免疫调节、消化、细胞保护、稳态和神经保护活性。已知可食用蘑菇在预防与年龄相关的疾病中发挥作用。几项研究表明，多糖、萜类化合物和酚类化合物是蘑菇衍生的具有药理活性的化学成分。由于用于蛋白质组学研究的蘑菇蛋白质提取的有效方案有限，关于这些与药用相关的蛋白质及其生物学功能的信息仍然不明。在此，我们对两种蘑菇（松萝）和（舞茸）进行了蛋白质组学研究。这些研究有助于揭示假定的蛋白质组相关神经保护过程的基础。从这两种蘑菇中回收的蛋白质显示出多种细胞特异性信号通路，包括未折叠蛋白反应、线粒体蛋白导入以及与BAG2、泛素化、细胞凋亡、微自噬、糖酵解、SNARE和免疫原性细胞信号通路相关的信号通路。这项研究发现了与蘑菇蛋白质组相关的蛋白质，有助于更好地了解用作替代药物具有广泛潜在健康益处的蘑菇的结构和功能特性。
