Liu Shuang, Yang Runmiao, Xu Jian
Key Laboratory of Bioorganic Synthesis of Zhejiang Department Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, P. R. China.
Chembiochem. 2025 Jul 18;26(14):e202500329. doi: 10.1002/cbic.202500329. Epub 2025 Jun 19.
Flavin-dependent ene-reductases (EREDs) have emerged as powerful biocatalysts for the asymmetric reduction of various substrates. This review focuses on the recent advances in light-induced electron transfer and subsequent reduction reactions mediated by EREDs. Upon photoexcitation, the flavin cofactor transitions to an excited state, significantly enhancing its reduction potential. Mechanistic insights into how light activation alters the redox properties of EREDs are discussed, leading to more efficient catalysis. The review also highlights the broadened application scope of photoexcited EREDs in organic synthesis. Additionally, the challenges and future directions in optimizing these light-driven biocatalytic processes are explored. This overview provides a foundation for developing novel, light-controlled enzymatic systems with enhanced catalytic performance.
黄素依赖性烯还原酶（EREDs）已成为用于各种底物不对称还原的强大生物催化剂。本综述重点关注光诱导电子转移以及由EREDs介导的后续还原反应的最新进展。光激发后，黄素辅因子转变为激发态，显著提高其还原电位。讨论了关于光激活如何改变EREDs氧化还原特性的机理见解，从而实现更高效的催化。该综述还强调了光激发的EREDs在有机合成中扩大的应用范围。此外，还探讨了优化这些光驱动生物催化过程中的挑战和未来方向。本概述为开发具有增强催化性能的新型光控酶系统奠定了基础。
