Li Zhenghui, Bian Xufei, Wu Huiyu, He Ling, Zeng Zuhua, Zhong Lei, Liu Yao, Li Yu, Hu Guihao, Mi Fanglin, Liu Zhen, Zhu Jiang
Medical Imaging Key Laboratory of Sichuan Province, North Sichuan Medical College, Nanchong, Sichuan, People's Republic of China.
Department of Stomatology, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, People's Republic of China.
Int J Nanomedicine. 2025 May 12;20:6043-6057. doi: 10.2147/IJN.S512565. eCollection 2025.
Phototherapy have gained significant traction in the treatment of tumors. However, the successful implementation of these therapies relies on photosensitizers with superior properties and precise guidance mechanisms.
In this study, we introduce an innovative method for the surface modification of titanium dioxide (TiO) nanoparticles through HRP-catalyzed covalent incorporation of Mn(II) chelate (Mn-Dopa) and dopamine.
This modification extends TiO nanoparticels' light absorption from ultraviolet to the near-infrared (NIR) range, endowing the nanoparticles with MRI-guided phototherapy capabilities. The resulting nanotheranostics system, TiO@PDA-MnDopa, demonstrated over 5-fold enhanced relaxivity compared to the monomeric MnDopa and exhibited synergistic phototherapy effects upon 808 nm laser excitation, with a photothermal conversion efficiency of 15.91%. In vitro and in vivo pharmacodynamics studies showed that the TiO@PDA-MnDopa demonstrated good safety in the HSC3 cell line and corresponding tumor-bearing mice, while effectively inhibiting tumor growth under 808 nm laser excitation.
This multifunctional nanotheranostic, integrating high relaxivity with synergistic PTT/PDT for MR imaging-guided phototherapy, holds great potential for applications in the early diagnosis, noninvasive treatment, and prognostic evaluation of oral squamous cell carcinoma.
光疗在肿瘤治疗中已获得显著关注。然而，这些疗法的成功实施依赖于具有优异性能的光敏剂和精确的引导机制。
在本研究中，我们介绍了一种通过辣根过氧化物酶催化的锰（II）螯合物（Mn-Dopa）和多巴胺的共价掺入对二氧化钛（TiO）纳米颗粒进行表面改性的创新方法。
这种改性将TiO纳米颗粒的光吸收从紫外扩展到近红外（NIR）范围，赋予纳米颗粒MRI引导的光疗能力。所得的纳米诊疗系统TiO@PDA-MnDopa与单体MnDopa相比，弛豫率提高了5倍以上，并在808nm激光激发下表现出协同光疗效果，光热转换效率为15.91%。体外和体内药效学研究表明，TiO@PDA-MnDopa在HSC3细胞系和相应的荷瘤小鼠中表现出良好的安全性，同时在808nm激光激发下有效抑制肿瘤生长。
这种多功能纳米诊疗剂将高弛豫率与协同光热疗法/光动力疗法相结合用于MR成像引导的光疗，在口腔鳞状细胞癌的早期诊断、无创治疗和预后评估中具有巨大的应用潜力。
