Wang Denian, Duan Yishang, He Lijuan, Jiang Juan, Xian Jinghong, Yuan Ke, Zhang Rui, Zhang Huohuo, Wang Jing, Li Nan, Huang Min, Hu Chenggong, Lu Sifeng, Luo Zhiheng, Deng Taibing, Zhang Zhongwei, Chen Bojiang, Li Weimin
Precision Medicine Research Center, Precision Medicine Key Laboratory of Sichuan Province, State Key Laboratory of Respiratory Health and Multimorbidity, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
Department of Respiratory and Critical Care Medicine, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
Commun Biol. 2025 May 26;8(1):804. doi: 10.1038/s42003-025-08234-1.
The interaction between gut and oropharyngeal microbiota plays a significant role in the viral infections like SARS-CoV-2, but role of the lower respiratory tract microbiota remains unclear. Our study utilized metatranscriptomics and metagenomics to analyze the microbial composition of bronchoalveolar lavage fluid and sputum samples from 116 COVID-19 patients, categorized into mild, severe, and critical groups. Our analysis revealed significant differences in viral genotypes across disease stages. As disease severity increased, the Chao index also rose. The mild group was predominantly dominated by Firmicutes, while the severe group showed an increase in Bacteroidetes. The critical group was characterized by a higher abundance of Actinobacteria and Proteobacteria. Notably, the abundance of Streptococcus and Rothia decreased as the disease progressed. Additionally, the Shannon index correlated with mortality risk, while the Chao index was associated with ICU admission, mechanical ventilation, and patient survival. These findings highlight the strong link between microbial composition and COVID-19 severity, providing valuable insights for assessing disease progression.
肠道微生物群与口咽微生物群之间的相互作用在诸如SARS-CoV-2等病毒感染中起着重要作用，但下呼吸道微生物群的作用仍不清楚。我们的研究利用宏转录组学和宏基因组学分析了116例COVID-19患者的支气管肺泡灌洗液和痰液样本的微生物组成，这些患者被分为轻症、重症和危重症组。我们的分析揭示了不同疾病阶段病毒基因型的显著差异。随着疾病严重程度的增加，Chao指数也上升。轻症组主要以厚壁菌门为主，而重症组拟杆菌门增加。危重症组的特征是放线菌门和变形菌门的丰度较高。值得注意的是，随着疾病进展，链球菌属和罗氏菌属的丰度下降。此外，香农指数与死亡风险相关，而Chao指数与入住重症监护病房、机械通气和患者生存相关。这些发现突出了微生物组成与COVID-19严重程度之间的紧密联系，为评估疾病进展提供了有价值的见解。
