Salmon Phil
Bruker Belgium (microCT), Kontich, Belgium.
Methods Mol Biol. 2025;2885:549-574. doi: 10.1007/978-1-0716-4306-8_27.
Micro-CT is becoming a gold standard for quantitative imaging in preclinical disease models in bone research. Osteoporosis research routinely uses microCT for 3D analysis of the architecture and mechanical competence of trabecular and cortical bone. While most published methodological research on microCT in bone has focused on osteoporosis, there is equal potential for microCT to quantitatively assess other bone disease models (and genetic phenotypes). Here a unified analysis approach or "pipeline" is presented for all preclinical bone research models, consisting of a series of steps: (i) the scan and reconstruction; (ii) standardized 3D image orientation; (iii) anatomically referenced volume of interest (VOI) delineation; (iv) segmentation and 3D analysis, with (v) application-specific interpretation of measured parameters. How this pipeline works in practice is described for several applications beyond osteoporosis (covered elsewhere in this volume): arthritis bone effects, arthritis cartilage effects, bone tumor, and fracture callus healing. All these analyses can be performed in a single software environment and morphometry pipeline.
微计算机断层扫描（Micro-CT）正成为骨研究中临床前疾病模型定量成像的金标准。骨质疏松症研究经常使用微计算机断层扫描对小梁骨和皮质骨的结构及力学性能进行三维分析。虽然大多数已发表的关于骨微计算机断层扫描的方法学研究都集中在骨质疏松症上，但微计算机断层扫描在定量评估其他骨疾病模型（以及基因表型）方面同样具有潜力。本文针对所有临床前骨研究模型提出了一种统一的分析方法或“流程”，该流程包括一系列步骤：（i）扫描与重建；（ii）标准化三维图像定向；（iii）基于解剖学的感兴趣体积（VOI）划定；（iv）分割与三维分析，以及（v）对测量参数进行特定应用的解读。本文针对骨质疏松症以外的几种应用（本卷其他地方有介绍）描述了该流程在实际中的工作方式：关节炎的骨效应、关节炎的软骨效应、骨肿瘤以及骨折痂愈合。所有这些分析都可以在单一软件环境和形态测量流程中进行。
