Evaluation of kefir consumption on gut microbial diversity in a healthy young population using full-length 16S rRNA sequencing.

INTRODUCTION

A balanced gut microbiota is essential for maintaining digestive, immune, and metabolic health. Kefir, a fermented milk beverage, influences gut microbiota through its probiotic composition and bioactive compounds, exhibiting various health-promoting effects. However, evidence on the effects of kefir on gut microbiota, particularly in healthy populations, is still limited. This study aimed to elucidate the effects of kefir on gut microbiota composition in healthy young adults under a controlled dietary environment.

METHODS

In this randomized, controlled, parallel-group trial, 28 healthy participants aged 18-30 years were assigned to one of three groups: kefir ( = 13), unfermented milk ( = 9), and yogurt ( = 6). Participants consumed 150 mL of their assigned beverage daily for 2 weeks. Stool samples were collected before and after the intervention to analyze gut microbiota composition using 16S rRNA sequencing.

RESULTS

Kefir consumption increased the relative abundance of lactate-producing bacteria, including , and . The genus also increased, with significant changes observed in and . These shifts in species abundance were associated with increases in the short-chain fatty acid (SCFA) production pathway.

DISCUSSION

In summary, this study highlights kefir's potential to modulate gut microbiota composition in healthy individuals, emphasizing its role in supporting gut health.